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Investigation of mechanisms involved in seed germination enhancement, enzymatic activity and seedling growth of rice (Oryza Sativa L.) using LPDBD (Ar+Air) plasma
Archives of Biochemistry and Biophysics ( IF 3.8 ) Pub Date : 2020-12-14 , DOI: 10.1016/j.abb.2020.108726
Mutasim Billah , Sumon Karmakar , Fahmida Begum Mina , Md. Nazmul Haque , Md. M. Rashid , Md. Faruk Hasan , Uzzal Kumar Acharjee , Mamunur Rashid Talukder

The study investigates the effect of LPDBD (Low Pressure Dielectric Barrier Discharge) (Ar + Air) plasma on seed germination, seedling growth and antioxidant enzyme activity of rice. Rice seeds were treated with LPDBD (Ar + Air) plasma for 2min, 4min, 6min, 8min and 10min. Seed germination rate, seedling growth, total chlorophyll content, enzymatic activity, total soluble sugar and protein concentration were increased in plants grown from the LPDBD (Ar + Air) plasma treated seeds. It was observed that the sprouting of seeds and the growth of seedlings of rice depends on the feed gases used to generate plasma and plasma processing time. In the case of plantlets germinated from the plasma-treated seeds of rice, the H2O2 level was increased significantly both in leaves and roots for 6min, 8min and 10min treatment respectively. No significant change was observed in Nitric Oxide (NO) concentration in seed, leaf, or root of plants grown from LPDBD (Ar + Air) plasma-treated seeds. The amount of total soluble sugar and protein increased significantly in the case of 2min, 4min, 6min, 8min and 10min seed treatment. Although plants exhibited no significant increase in APX activities, but a significant increase of CAT and SOD activity in the leaf and root was found. This study reveals that LPDBD (Ar + Air) plasma is involved in the elevation of ROS species in leaf and root of rice plants which is tightly regulated by the upregulation of CAT activity that ultimately enhances the seed germination and growth of rice plantlets.



中文翻译:

利用LPDBD(Ar + Air)血浆研究水稻(Oryza Sativa L.)种子的发芽增强,酶活性和幼苗生长的机制

这项研究调查了LPDBD(低压介质阻挡放电)(Ar +空气)血浆对水稻种子发芽,幼苗生长和抗氧化酶活性的影响。水稻种子用LPDBD(Ar + Air)血浆处理2min,4min,6min,8min和10min。用LPDBD(Ar + Air)血浆处理过的种子种植的植物,其种子发芽率,幼苗生长,总叶绿素含量,酶活性,总可溶性糖和蛋白质浓度均增加。据观察,水稻的种子发芽和幼苗的生长取决于用于产生血浆的原料气和血浆处理时间。对于从经过等离子体处理的水稻种子发芽的小植株,H 2 O 2分别在叶片和根部处理6min,8min和10min均显着提高了其水平。用LPDBD(Ar +空气)等离子体处理过的种子生长的植物的种子,叶片或根中的一氧化氮(NO)浓度未见明显变化。在种子处理2min,4min,6min,8min和10min的情况下,可溶性糖和蛋白质的总量显着增加。虽然植物的APX活性没有显着增加,但是叶和根中CAT和SOD活性却显着增加。这项研究表明,LPDBD(Ar +空气)血浆参与了水稻叶片和根中ROS种类的升高,而CAT活性的上调严格地调节了ROS的活性,最终增强了水稻幼苗的发芽和生长。

更新日期:2020-12-23
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