Functional cartilage tissue engineering needs a substantial, easy to handle scaffold with proper mechanical strength to repair defected area in articular cartilage. In this study, we report the development and characterization of demineralized bone matrix (DBM) in with a poly vinyl alcohol (PVA) to have a proper homogenous injectable scaffold. Injectabiliy of the biodegradable scaffolds, degradation rate, swelling ratio compression and tensile mechanical properties, and viability and proliferation of bone marrow mesenchymal stem cells (BM-MSCs) followed by differentiation of them In-vitro and In-vivo seeded within the scaffold were studied. It demonstrated that the PVA 20% could increase significantly (p < 0.05) the biodegradability of DBM after 720 hours.DBM with 20% of PVA scaffold has significantly higher (p < 0.05) compression and tensile mechanical strength and viscosity. SEM images showed a multilayer of cells on DBM scaffold incorporated with PVA 20%.BM-MSCs on scaffolds, DBM+PVA 20% had a significant growth rate (p < 0.0001) compare to 2D and low concentration of PVA after 21 days of culture. Viability of cells was significantly higher (p < 0.05) on DBM+PVA scaffold compare to DBM. DBM+PVA 20% enhanced cell viability (P < 0.05) compare to DBM scaffold. The PVA presence enhanced chondrogenesis differentiation at the cellular and molecular levels, as evidenced by increased COL II (P < 0.05) and SOX2 upregulation of Chondrogensis-specific genes (p < 0.001). Hyline-like cartilage covered the defect which was confirmed by microscopy and histology assessments. Having considered percentages of PVA with a constant amount of DBM, injectability, compressive mechanical properties, homogeneity of the scaffold, and providing sufficient surface area (12.25 cm²/ml) for cell attachment; 0.35 g/ml of DBM in 20% PVA (w/v) has applicable properties within the ranges of studies which can be proposed for the injectable engineered articular cartilage.

功能性软骨组织工程需要具有适当机械强度的大量、易于处理的支架来修复关节软骨中的缺陷区域。在这项研究中，我们报告了脱矿质骨基质 (DBM) 与聚乙烯醇 (PVA) 的开发和表征，以获得适当的均质可注射支架。研究了可生物降解支架的可注射性、降解速率、膨胀比压缩和拉伸力学性能，以及骨髓间充质干细胞 (BM-MSCs) 的活力和增殖，然后在支架内进行体外和体内接种. 结果表明，20% PVA 可显着提高 DBM 在 720 小时后的生物降解性（p < 0.05）。20% PVA 支架的 DBM 显着提高（p < 0.05）。05) 压缩和拉伸机械强度和粘度。SEM 图像显示 DBM 支架上的多层细胞与 PVA 20% 结合。支架上的 BM-MSCs，DBM+PVA 20% 与 2D 相比具有显着的生长速率 (p < 0.0001)，培养 21 天后 PVA 浓度低. 与 DBM 相比，DBM+PVA 支架上的细胞活力明显更高（p < 0.05）。与 DBM 支架相比，DBM+PVA 提高了 20% 的细胞活力 (P < 0.05)。PVA 的存在增强了细胞和分子水平上的软骨形成分化，这可以通过增加 与 DBM 相比，DBM+PVA 支架上的细胞活力明显更高（p < 0.05）。与 DBM 支架相比，DBM+PVA 提高了 20% 的细胞活力 (P < 0.05)。PVA 的存在增强了细胞和分子水平上的软骨形成分化，这可以通过增加 与 DBM 相比，DBM+PVA 支架上的细胞活力明显更高（p < 0.05）。与 DBM 支架相比，DBM+PVA 提高了 20% 的细胞活力 (P < 0.05)。PVA 的存在增强了细胞和分子水平上的软骨形成分化，这可以通过增加COL II (P < 0.05) 和SOX2上调 Chondrogensis 特异性基因 (p < 0.001)。Hyline 样软骨覆盖了通过显微镜和组织学评估证实的缺陷。考虑了具有恒定 DBM 量的 PVA 百分比、可注射性、压缩机械性能、支架的均匀性以及为细胞附着提供足够的表面积（12.25 cm ² /ml）；20% PVA (w/v) 中的 0.35 g/ml DBM 在可用于可注射工程关节软骨的研究范围内具有适用特性。