Functional mapping of photoreceptor physiology is important for better disease diagnosis and treatment assessment. Fast intrinsic optical signal (IOS), which arises before light-evoked pupillary response, promises a unique biomarker of photoreceptor physiology for objective optoretinography with high resolution. This study is to test the feasibility of non-mydriatic IOS mapping of retinal photoreceptors in awake human. Depth-resolved optical coherence tomography verified outer segment (OS) as the anatomic origin of fast photoreceptor-IOS. Dynamic IOS changes are primarily confined at OS boundaries connected with inner segment and retinal pigment epithelium, supporting transient OS shrinkage due to phototransduction process as the mechanism of the fast photoreceptor-IOS response.

光感受器生理学的功能图谱对于更好的疾病诊断和治疗评估非常重要。在光诱发瞳孔反应之前产生的快速固有光信号（IOS）有望成为高分辨率客观视视网膜成像的光感受器生理学的独特生物标志物。本研究旨在测试清醒人视网膜感光器免散瞳 IOS 映射的可行性。深度分辨光学相干断层扫描证实外节 (OS) 是快速光感受器 IOS 的解剖起源。动态 IOS 变化主要局限于与内节和视网膜色素上皮相连的 OS 边界，支持由于光转导过程作为快速光感受器 IOS 响应机制而导致的短暂 OS 收缩。