Since the discovery of their toxicity to aquatic environments, antifouling booster biocides (ABBs) have been widely researched and detected at trace levels in diverse environmental compartments including water, sediment, and, less frequently, biota. Hence, the reliable assessment of environmental risks posed by ABBs requires the development of analytical methods sufficiently robust, accurate, and precise for the simultaneous trace-level determination of ABBs. Herein, we summarize outstanding sample preparation procedures for the analysis of main ABBs in environmental matrices, describing techniques ranging from traditional extraction methods to novel miniaturized and micro-extraction ones, which have recently received much attention due to their reduced number of steps, low operational cost, and greater respect for the environment. The main applied chromatographic-based methods coupled to different detection techniques are also addressed. Despite the recent development of numerous ABBs determination methods, this topic continues to draw attention because of the lack of standardization among methods, despite legislation set up maximum standards levels for selected ABBs, and the need to monitor ABB transformation products for a reliable ecological risk assessment.

自发现其对水生环境的毒性以来，防污增强型杀生物剂（ABB）已在包括水，沉积物以及较不常见的生物区系在内的各种不同环境隔间中以痕量水平进行了广泛研究和检测。因此，对ABB构成的环境风险进行可靠的评估需要开发出足够健壮，准确和精确的分析方法，以便同时测定ABB的痕量水平。在此，我们总结了用于分析环境基质中主要ABB的出色样品制备程序，描述了从传统提取方法到新颖的小型化和微萃取方法等技术，这些技术最近因其步骤数减少，操作少而备受关注成本，并更加尊重环境。还讨论了与不同检测技术相结合的主要基于色谱的方法。尽管最近已经开发了许多ABB测定方法，但是尽管立法为选定的ABB设置了最高标准水平，并且需要监控ABB转化产品以进行可靠的生态风险评估，但由于方法之间缺乏标准化，因此该主题继续受到关注。 。