Insect microbiota are receiving increasing attention from researchers, particularly with the continued advances in next generation sequencing (NGS) techniques. However, there is a paucity of data on the microbiota of ants that scavenge around human settlements. In this study, we characterized the bacterial communities of Pheidole rugaticeps Emery that were collected scavenging on other household insects using Illumina MiSeq high-throughput sequencing of the bacterial 16S ribosomal DNA gene. P. rugaticeps DNA was extracted from the insect samples using a HiYield™ Genomic DNA isolation kit according to the manufacturer’s protocols and amplified using polymerase chain reaction (PCR). The PCR products were sequenced with the Illumina MiSeq platform according to the standard protocols to amplify the V3–V4 of the 16S rDNA gene. The results for the 16S rDNA genes were analysed using QIIME 2 Core − 2020.6, and a 16S rDNA metabarcoding dataset was presented. A total of 46,651 reads were obtained from three genomic samples. A total of 368 amplicon sequence variants (ASV) comprising 165 genera were revealed and classified into 17 phyla. Proteobacteria (57.47%) and Firmicutes (33.14%) were the most abundant taxa, while Acinetobacter (37.10%) was the most abundant genus in all three sampling groups. Pathogenic bacteria species, such as Acinetobacter baumannii (15%) and Pseudomonas aeruginosa (2.92%), were identified from P. rugaticeps samples collected from a hospital environment. However, this study recommends more studies on the microbiota of Pheidole ants with different feeding habits and habitats to establish their core microbiome.

昆虫微生物群正受到研究人员的越来越多的关注，特别是随着下一代测序（NGS）技术的不断发展。但是，关于在人类住区周围清扫的蚂蚁微生物群的数据很少。在这项研究中，我们表征了使用Illumina MiSeq高通量测序细菌16S核糖体DNA基因在其他家庭昆虫身上清除的搜集到的Pheidole rugaticeps Emery细菌群落。皱纹疟原虫根据制造商的规程，使用HiYield™Genomic DNA分离试剂盒从昆虫样品中提取DNA，并使用聚合酶链反应（PCR）进行扩增。根据标准方案，使用Illumina MiSeq平台对PCR产物进行测序，以扩增16S rDNA基因的V3-V4。使用QIIME 2 Core-2020.6分析了16S rDNA基因的结果，并提出了16S rDNA元条形码数据集。从三个基因组样本中总共获得46,651个读数。揭示了总共165个属的368个扩增子序列变体（ASV），并将其分类为17个门。变形杆菌（57.47％）和Firmicutes（33.14％）是最丰富的分类单元，而不动杆菌（37.10％）是所有三个采样组中最丰富的属。从医院环境中收集到的P. rugaticeps样本中鉴定出病原细菌，例如鲍曼不动杆菌（15％）和铜绿假单胞菌（2.92％）。但是，这项研究建议对具有不同摄食习惯和生境的费托蚂蚁的微生物群进行更多的研究，以建立其核心微生物组。