Here, 38 wheat PYL genes (TaPYLs) belonging to 13 homoeologous groups were identified using the genome-search method, with 26 and 12 PYL genes identified in Triticum dicoccoides and Aegilops tauschii, respectively. Phylogenetic relationship, conserved domain and molecular evolution analysis revealed that PYL genes showed highly conservative between wheat and theprogenitors. Interaction network and miRNA target prediction found that TaPYLs could interact with the important components of ABA signaling pathway and Tae-miR966b-3p might be a hub regulator mediating wheat ABA signal network. Furthermore, the tissue-specific and stress-responsive TaPYLs were detected through RNA-seq analysis. Expressions of 10 TaPYLs were validated by QPCR analysis and the homoeologous genes showed significantly differential expression, suggesting subfunctionalization of them has occurred. Finally, 3D structures of the TaPYL proteins were predicted by homology modeling. This study lays the foundation for further functional study of PYL genes for development and stress tolerance improvement in wheat and beyond.

在这里，使用基因组搜索方法鉴定了属于 13 个同源组的38 个小麦 PYL 基因（TaPYL s），其中在Triticum dicoccoides和Aegilops tauschii 中鉴定了 26 个和 12 个 PYL 基因，分别。系统发育关系、保守结构域和分子进化分析表明，PYL基因在小麦和祖代之间表现出高度保守性。相互作用网络和miRNA靶点预测发现TaPYLs可以与ABA信号通路的重要成分相互作用，Tae-miR966b-3p可能是介导小麦ABA信号网络的中枢调节因子。此外，通过 RNA-seq 分析检测到组织特异性和应激反应性 TaPYL。通过QPCR分析验证了10个TaPYLs的表达，同源基因表现出显着差异表达，表明它们发生了亚功能化。最后，通过同源建模预测了 TaPYL 蛋白的 3D 结构。