The municipal and agricultural wastes are promising source of ligniocellulosic biomasses (LBs) for bioethanol production. However, the pretreatment-hydrolysis (P–H) of LB is the most difficult process in bioethanol production due to high crystallinity of LB compositional structures. In this study, p-toluenesulphonic acid (PTSA) was investigated for P–H of multi-substrate (MS) sample of LBs. Five alternative MS samples were prepared from 10 single LBs. Based on the total reducing sugar yield, one of the MS was selected for optimization experiment. The factors affecting P–H (acid concentration, time and temperature) were optimized to compare catalytic activity of PTSA and H₂SO₄. Under the optimized conditions, maximum total reducing sugar (TRS) yield of 95.89 and 78.26% was obtained using PTSA and H₂SO₄, respectively. Mass production of hydrolysate was carried out using both catalysts at a substrate load of 50 g.L⁻¹ and fermented at an optimum yeast load of 10% (v/v). The ethanol yield from the hydrolysates of PTSA treatment was 16.89 g L⁻¹ (74.5%) and from H₂SO₄ was 13.17 g L⁻¹ (70.9%) at 48 & 56 h fermentation time, respectively. The PTSA surpassed the conventional method of treatment (H₂SO₄) both in TRS and ethanol yield. The efficiency of PTSA can be noticed in that one third of the acid concentration required in sulfuric acid treatment was used in PTSA to obtain 96% of TRS at lower temperature. This study revealed the potential of aqueous PTSA for the hydrolysis of a mixture of feedstocks which is much more complex and challenging than treating a single substrate.

城市和农业废物是用于生物乙醇生产的木质纤维素生物质（LBs）的有前途的来源。但是，由于LB组成结构的高结晶度，LB的预处理-水解（PH）是生物乙醇生产中最困难的过程。在这项研究中，对甲苯磺酸（PTSA）用于LB的多底物（MS）样品的PH进行了研究。从10个单个LB制备了五个替代MS样品。根据总还原糖产量，选择其中一种质谱进行优化实验。优化了影响PH的因素（酸浓度，时间和温度）以比较PTSA和H ₂ SO _4的催化活性。在最佳条件下，使用PTSA和H ₂ SO ₄可获得的最大总还原糖（TRS）产率分别为95.89和78.26％。使用两种催化剂以50 gL ^-1的底物负载量大规模生产水解产物，并以10％（v / v）的最佳酵母负载量发酵。在发酵48和56小时时，来自PTSA处理的水解产物的乙醇产量分别为16.89 g L ^-1（74.5％）和来自H ₂ SO ₄的乙醇产量为13.17 g L ^-1（70.9％）。PTSA超越了传统的处理方法（H ₂ SO ₄）的TRS和乙醇收率。可以注意到PTSA的效率，因为在PTSA中使用了硫酸处理所需酸浓度的三分之一来在较低的温度下获得96％的TRS。这项研究揭示了PTSA水溶液水解原料混合物的潜力，这比处理单一底物要复杂得多且更具挑战性。