Research on the effect of pulsed electric field in cell electroporation attracts more attention, especially tumor ablation. This puts forward higher requirements for pulsed electric field parameters. The shape of the pulse waveform was also required in different electroporation applications. Based on this, a multilevel pulse generator with series capacitor structure was proposed in this article. Besides, it can also obtain the different shapes of the pulse waveforms by controlling the timing of semiconductor switches. The voltage amplitude, the number of pulses, and the pulsewidth are all adjustable. A ten-level test prototype with 11 switches was built. The structure was simple and was more compact. It can work stably under the simulated small resistance load. Finally, when the dc charging voltage was 1 kV, the system can achieve a maximum output voltage of ±500 V. The number of pulse in one pulse sequence was 1–500. The pulsewidth was adjustable from 1 to $10~\mu \text{s}$ . By controlling the switch’s timing, it can also obtain the multilevel voltage output, exponential waveform voltage output, and composite waveform output combined narrow pulse with wide pulse.

中文翻译：

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基于串联电容器结构的细胞电穿孔多电平脉冲发生器

脉冲电场在细胞电穿孔中的作用研究越来越受到关注，尤其是肿瘤消融。这就对脉冲电场参数提出了更高的要求。在不同的电穿孔应用中也需要脉冲波形的形状。基于此，本文提出了一种串联电容结构的多电平脉冲发生器。此外，它还可以通过控制半导体开关的时序来获得不同形状的脉冲波形。电压幅值、脉冲数、脉宽均可调。构建了具有 11 个开关的十级测试原型。结构简单，更加紧凑。可在模拟小电阻负载下稳定工作。最后，当直流充电电压为 1 kV 时，系统最大输出电压可达±500 V。一个脉冲序列中的脉冲数为1-500。脉冲宽度从 1 到 $10~\mu \text{s}$ 可调。通过控制开关的时序，还可以获得多电平电压输出、指数波形电压输出、窄脉冲和宽脉冲组合的复合波形输出。