The first autosomal sequence‐based allele (aka SNP‐STR haplotype) frequency database for forensic massively parallel sequencing (MPS) has been published, thereby removing one of the remaining barriers to implementing MPS in casework. The database was developed using a specific set of flank trim sites. If different trim sites or different kits with different primers are used for casework, then SNP‐STR haplotypes may be detected that do not have frequencies in the database. We describe a procedure to address calculation of match probabilities when casework samples are generated using an MPS kit with different trim sites than those present in the relevant population frequency database. The procedure provides a framework for comparison of any MPS kit or database combination while also accommodating comparison of MPS and CE profiles.

中文翻译：

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法医 PCR-mps 试剂盒中基于序列的等位基因的匹配统计数据

第一个用于法医大规模并行测序 (MPS) 的基于常染色体序列的等位基因（又名 SNP-STR 单倍型）频率数据库已经发布，从而消除了在案件中实施 MPS 的剩余障碍之一。该数据库是使用一组特定的侧翼修剪站点开发的。如果将不同的修剪位点或具有不同引物的不同试剂盒用于个案工作，则可能会检测到数据库中没有频率的 SNP-STR 单倍型。我们描述了一个程序来解决匹配概率的计算，当个案工作样本是使用 MPS 套件生成的，其修剪点与相关人口频率数据库中存在的修剪点不同。该过程提供了一个框架，用于比较任何 MPS 套件或数据库组合，同时还可以比较 MPS 和 CE 配置文件。