Molecular detection and characterization of Theileria sp. from hedgehogs ( Paraechinus aethiopicus ) in Saudi Arabia,Letters in Applied Microbiology

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Molecular detection and characterization of Theileria sp. from hedgehogs ( Paraechinus aethiopicus ) in Saudi Arabia
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-12-31 , DOI: 10.1111/lam.13438
O.B. Mohammed ₁ , N.M.S. Amor _{1,

2} , S.A. Omer ₃ , A.N. Alagaili ₁

Affiliation

In this study, we conducted molecular detection and characterization of piroplasms that infect the Ethiopian or desert hedgehogs (Paraechinus aethiopicus) in Saudi Arabia. Blood samples from 112 (68 males and 44 females) desert hedgehogs from Unaizah, Central Saudi Arabia were screened for Theileria/Babesia DNA using the polymerase chain reaction (PCR) employing specific primers amplifying the partial 18S small subunit rRNA gene. Theileria DNA was detected in 51 samples (54.5%), giving a prevalence of 45.5%. Theileria DNA was found in 33 (48.5%) males and 18 (40.9%) females, and there was no significant difference (p > 0.05) in the prevalence between males and females. Similarly, there was no significant difference (p > 0.05) in the prevalence between juveniles (40%) and adults (46.7%). There was a significant difference in the prevalence of Theileria in hedgehogs collected from May to September and the period from October to April (p = 0.003). Four haplotypes of Theileria sp. in hedgehogs were detected and designated as H1 to H4. H1 was the predominant haplotype and found in 80.8% of the positive individuals. Partial sequences of the 18S rRNA of Theileria sp. from hedgehogs grouped with Theileria spp. that are benign. This study is the first report of the occurrence of Theileria spp. in Saudi Arabian desert hedgehogs.

中文翻译：

泰勒氏菌的分子检测和表征。来自沙特阿拉伯的刺猬 ( Paraechinus aethiopicus )

在这项研究中，我们对感染沙特阿拉伯的埃塞俄比亚或沙漠刺猬（Paraechinus aethiopicus）的梨浆进行了分子检测和表征。使用聚合酶链反应 (PCR) 使用扩增部分 18S 小亚基 rRNA 基因的特异性引物，对来自沙特阿拉伯中部 Unaizah 的 112 只沙漠刺猬（68 只雄性和 44 只雌性）的血液样本进行泰勒虫/巴贝虫 DNA 筛选。在 51 个样本 (54.5%) 中检测到泰勒虫 DNA，患病率为 45.5%。在 33 名（48.5%）男性和 18 名（40.9%）女性中发现泰勒虫 DNA，男女患病率无显着差异（p > 0.05）。同样，青少年 (40%) 和成人 (46.7%) 的患病率也没有显着差异 (p > 0.05)。5-9 月和 10-4 月期间采集的刺猬中泰勒氏菌的患病率存在显着差异（p = 0.003）。泰勒虫属的四种单倍型。在刺猬中检测到并命名为 H1 至 H4。H1 是主要的单倍型，在 80.8% 的阳性个体中发现。泰勒虫 18S rRNA 的部分序列。来自与泰勒虫属分组的刺猬。是良性的。本研究是第一份关于泰勒虫发生的报告。在沙特阿拉伯沙漠刺猬中。泰勒虫 18S rRNA 的部分序列。来自与泰勒虫属分组的刺猬。是良性的。本研究是第一份关于泰勒虫发生的报告。在沙特阿拉伯沙漠刺猬中。泰勒虫 18S rRNA 的部分序列。来自与泰勒虫属分组的刺猬。是良性的。本研究是第一份关于泰勒虫发生的报告。在沙特阿拉伯沙漠刺猬中。

更新日期：2020-12-31

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