Debaryomyces hansenii is traditionally described as a halotolerant non‐conventional yeast and has served as a model organism for the study of osmotolerance and salt tolerance mechanisms in eukaryotic systems for the past 30 years. However, unraveling of D. hansenii's biotechnological potential has always been difficult due to the persistent limitations in the availability of efficient molecular tools described for this yeast. Additionally, there is a lack of consensus and contradictory information along the recent years that limits a comprehensive understanding of its central carbon metabolism, mainly due to a lack of physiological studies in controlled and monitored environments. Moreover, there is little consistency in the culture conditions (media composition, temperature, and pH among others) used by different groups, which makes it complicated when trying to get prevalent conclusions on behavioral patterns. In this work, we present for the first time a characterization of D. hansenii in batch cultivations using highly controlled lab‐scale bioreactors. Our findings contribute to a more complete picture of the central carbon metabolism and the external pH influence on the yeast's ability to tolerate high Na⁺ and K⁺ concentrations, pointing to a differential effect of both salts, as well as a positive effect in cell performance when low environmental pH values are combined with a high sodium concentration in the media. Finally, a novel survival strategy at very high salinity (2 M) is proposed for this yeast, as well as potential outcomes for its use in industrial biotechnology applications.

中文翻译：

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受控生物反应器中的生理特征揭示了汉森德巴利酵母在高盐度下的新生存策略

Debaryomyces hansenii传统上被描述为一种耐盐的非常规酵母，在过去 30 年中一直作为研究真核系统中渗透压和耐盐机制的模式生物。然而，D. hansenii 的解体由于针对这种酵母描述的有效分子工具的可用性持续受到限制，因此其生物技术潜力一直很困难。此外，近年来缺乏共识和矛盾的信息，限制了对其中心碳代谢的全面了解，这主要是由于缺乏在受控和监测环境中的生理学研究。此外，不同群体使用的培养条件（培养基成分、温度和 pH 值等）几乎没有一致性，这使得在尝试对行为模式得出普遍结论时变得复杂。在这项工作中，我们首次介绍了D. hansenii使用高度控制的实验室规模生物反应器分批培养。我们的发现有助于更全面地了解中央碳代谢和外部 pH 值对酵母耐受高 Na ⁺和 K ⁺浓度能力的影响，指出两种盐的不同作用，以及对细胞性能的积极影响当低环境 pH 值与介质中的高钠浓度相结合时。最后，针对这种酵母提出了一种在极高盐度 (2 M) 下的新型生存策略，以及将其用于工业生物技术应用的潜在结果。