Vogt–Koyanagi–Harada patients show higher frequencies of circulating NKG2Dpos NK and NK T cells,Clinical & Experimental Immunology

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Vogt–Koyanagi–Harada patients show higher frequencies of circulating NKG2Dpos NK and NK T cells
Clinical & Experimental Immunology ( IF 3.4 ) Pub Date : 2020-12-12 , DOI: 10.1111/cei.13563
M Bonacini ₁ , L Cimino ₂ , L De Simone ₂ , E Bolletta ₂ , F Gozzi ₂ , A Soriano ₃ , F Muratore ₃ , A Zerbini ₁ , L Fontana ₄ , C Salvarani _{3,

5} , S Croci ₁

Affiliation

Vogt–Koyanagi–Harada (VKH) is an autoimmune disease characterized by inflammation in tissues that contain melanocytes. We aimed to increase the knowledge regarding immunological pathways deregulated in VKH disease. We compared the percentages of circulating natural killer (NK), NK T and T cells expressing the activatory markers: CD16, CD69, NK group 2D (NKG2D), natural cytotoxicity triggering receptor 3 (Nkp30), natural cytotoxicity triggering receptor 1 (Nkp46) and the inhibitory marker: NK group 2 member A (NKG2A) in 10 active VKH patients, 20 control subjects (CTR) and seven patients with Behçet disease (BD) by flow cytometry. Cytotoxic potential of NK cells was determined through the degranulation marker CD107a expression after contact with K562 cells by flow cytometry. Moreover, plasmatic levels of 27 cytokines were determined with a multiplex bead‐based assay. VKH patients showed higher percentages of NKG2D^pos NK and NK T cells versus CTR. The cytotoxic potential of NK cells induced by K562 cells was comparable between VKH patients and CTR. Finally, higher concentrations of interleukin (IL)‐4, IL‐5, IL‐7, IL‐17 and platelet‐derived growth factor‐subunits B (PDGF‐bb) were detected in plasma of VKH patients versus CTR. The immune profile of VKH patients was similar to that of BD patients.

中文翻译：

Vogt-Koyanagi-Harada 患者表现出更高频率的循环 NKG2Dpos NK 和 NK T 细胞

Vogt-Koyanagi-Harada (VKH) 是一种自身免疫性疾病，其特征是含有黑色素细胞的组织发生炎症。我们旨在增加关于 VKH 疾病中免疫通路失调的知识。我们比较了循环自然杀伤 (NK)、NK T 和 T 细胞表达激活标志物的百分比：CD16、CD69、NK 组 2D (NKG2D)、天然细胞毒性触发受体 3 (Nkp30)、天然细胞毒性触发受体 1 (Nkp46)和抑制标志物：流式细胞仪检测 10 名活动性 VKH 患者、20 名对照受试者 (CTR) 和 7 名白塞病 (BD) 患者中的 NK 组 2 成员 A (NKG2A)。通过流式细胞术与 K562 细胞接触后，通过脱粒标记 CD107a 表达确定 NK 细胞的细胞毒性潜力。而且，27 种细胞因子的血浆水平是通过多重微珠测定法测定的。VKH 患者的 NKG2D 百分比更高^pos NK 和 NK T 细胞与CTR。K562 细胞诱导的 NK 细胞的细胞毒性潜能在 VKH 患者和 CTR 之间相当。最后，与CTR相比，在 VKH 患者的血浆中检测到更高浓度的白细胞介素 (IL)-4、IL-5、IL-7、IL-17 和血小板衍生生长因子-亚基 B (PDGF-bb) 。VKH 患者的免疫特征与 BD 患者相似。

更新日期：2020-12-12

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