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Proposal of a laboratory course dedicated to the generation of protein molecular weight standards for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis
Biochemistry and Molecular Biology Education ( IF 1.2 ) Pub Date : 2020-12-10 , DOI: 10.1002/bmb.21476
José Bubis 1
Affiliation  

Protein molecular weight standards are routinely employed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the apparent sizes of unknown proteins within a sample. For training students, a laboratory course based on the production of marker proteins is proposed. Following fractionation by column chromatography, a series of purified proteins and mixtures of proteins were combined to generate various sets of unstained, prestained and fluorescently labeled molecular weight ladders for SDS-PAGE. The produced material can be used in successive laboratory practices as an alternative to commercial protein markers.

中文翻译:

致力于为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳建立蛋白质分子量标准的实验室课程提案

十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 中通常使用蛋白质分子量标准来估计样品中未知蛋白质的表观大小。对于培训学生,建议开设基于标记蛋白生产的实验室课程。在通过柱层析分馏之后,将一系列纯化的蛋白质和蛋白质混合物组合起来,生成各种用于 SDS-PAGE 的未染色、预染色和荧光标记的分子量阶梯。生产的材料可用于连续的实验室实践,作为商业蛋白质标记的替代品。
更新日期:2020-12-10
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