Protein molecular weight standards are routinely employed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the apparent sizes of unknown proteins within a sample. For training students, a laboratory course based on the production of marker proteins is proposed. Following fractionation by column chromatography, a series of purified proteins and mixtures of proteins were combined to generate various sets of unstained, prestained and fluorescently labeled molecular weight ladders for SDS-PAGE. The produced material can be used in successive laboratory practices as an alternative to commercial protein markers.

中文翻译：

---

致力于为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳建立蛋白质分子量标准的实验室课程提案

十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 中通常使用蛋白质分子量标准来估计样品中未知蛋白质的表观大小。对于培训学生，建议开设基于标记蛋白生产的实验室课程。在通过柱层析分馏之后，将一系列纯化的蛋白质和蛋白质混合物组合起来，生成各种用于 SDS-PAGE 的未染色、预染色和荧光标记的分子量阶梯。生产的材料可用于连续的实验室实践，作为商业蛋白质标记的替代品。