Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) is a plant-specific serine/threonine kinase involved in response to adverse environmental stimuli. Previous studies showed that TaSnRK2.4 was involved in response to abiotic stresses and conferred enhanced tolerance to multiple stresses in Arabidopsis. Further experiments were performed to decipher the underlying mechanisms and discover new functions. The genomic sequences of TaSnRK2.4s locating on chromosome 3A, 3B and 3D were obtained. Sequencing identified one and 13 variations of TaSnRK2.4–3A and TaSnRK2.4-3B, respectively, but no variation was detected in TaSnRK2.4-3D. The markers 2.4AM1, 2.4BM1 and 2.4BM2 were developed based on three variations. Association analysis showed that both TaSnRK2.4-3A and TaSnRK2.4-3B were significantly associated with thousand-kernel weight (TKW), and that SNP3A-T and SNP3B-C were favorable alleles for higher TKW. Yeast two-hybrid and split luciferase assays showed that TaSnRK2.4 physically interacted with abiotic stress responsive protein TaLTP3, suggesting that TaSnRK2.4 enhanced abiotic stress tolerance by activating TaLTP3. Our studies suggested that TaSnRK2.4 have potential in improving TKW and response to abiotic stress.

蔗糖非发酵型1相关蛋白激酶2（SnRK2）是一种植物特异性丝氨酸/苏氨酸激酶，参与对不良环境刺激的反应。先前的研究表明，TaSnRK2.4参与了对非生物胁迫的响应，并增强了拟南芥对多种胁迫的耐受性。进行了进一步的实验以破译潜在的机制并发现新的功能。获得了位于3A，3B和3D染色体上的TaSnRK2.4s的基因组序列。测序分别鉴定出TaSnRK2.4-3A和TaSnRK2.4-3B的一种和13种变异，但在TaSnRK2.4-3D中未检测到变异。标记2.4AM1、2.4BM1和2.4BM2是根据三个变体开发的。关联分析表明，TaSnRK2.4-3A和TaSnRK2.4-3B与千粒重（TKW）显着相关，而SNP3A-T和SNP3B-C是较高TKW的有利等位基因。酵母两杂交和拆分荧光素酶测定表明TaSnRK2.4与非生物胁迫响应蛋白TaLTP3物理相互作用，表明TaSnRK2.4通过激活TaLTP3增强了非生物胁迫耐受性。我们的研究表明TaSnRK2.4具有改善TKW和应对非生物胁迫的潜力。