Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F1 hybrid was obtained by crossing the Silihong (Slh) cultivar with Arachis duranensis (ZW55), a diploid wild species. Seedlings were generated by embryo rescue and tissue culture. A true interspecific hybrid was then confirmed by cytological methods and molecular markers. After treating seedlings with colchicine during in vitro multiplication, the established interspecific F1 hybrid produced seeds which were named as Am1210. With oligonucleotide fluorescence in situ hybridization (Oligo FISH), molecular marker evaluations, morphological and web blotch resistance characterization, we found that: 1) Am1210 was an allohexaploid between Slh and ZW55; 2) the traits of spreading lateral branches, single-seeded or double-seeded pods and red seed coats were observed to be dominant compared to the erect type, multiple-seeded pods and brown seed coats; 3) the web blotch resistance of Am1210 was significantly improved than that of Slh, indicating the contribution of the web blotch resistance from the wild parent A. duranensis. In addition, 69 dominant and co-dominant molecular markers were developed which could be both used to verify the hybrid in this study and to identify translocation or introgression lines with A. duranensis chromosome fragments in future studies as well.

花生病严重威胁花生生产，通过种间杂交产生抗病材料是解决这一问题的有效方法。在这项研究中，通过将Silihong（Slh）品种与二倍体野生种Arachis duranensis（ZW55）杂交，获得了种间F1杂种的胚。通过胚胎抢救和组织培养产生幼苗。然后通过细胞学方法和分子标记确定了真正的种间杂种。在体外增殖过程中用秋水仙碱处理幼苗后，建立的种间F1杂种产生了被称为Am1210的种子。寡核苷酸原位荧光杂交（Oligo FISH），分子标记物评估，形态学和抗网斑的特性，我们发现：1）Am1210是Slh和ZW55之间的异六倍体；2）与直立型，多种子荚和棕色种皮相比，侧枝蔓延，单种或双种荚和红色种皮的性状占主导。3）Am1210的网斑病电阻比SLH的被显著改善，指示网斑病抗性的野生亲本贡献A. duranensis。此外，还开发了69个显性和共显性分子标记，可用于验证本研究中的杂种并鉴定杜伦曲霉的易位或渗入系 染色体碎片也将在未来的研究中。