Radish (Raphanus sativus L.), an important root vegetable crop of the Brassicaceae family, has a high level of anthocyanin accumulation in its pigment root tissues. It was reported that MYB transcription factors (TFs) play vital roles in plant development and anthocyanin metabolism, and the PAP1/2 could promote expression of anthocyanin biosynthesis genes. In this study, a total of 187 radish MYB genes (RsMYBs) were identified in the radish genome and clustered into 32 subfamilies. Among them, 159 RsMYBs were localized on nine radish chromosomes. Interestingly, 14 RsMYBs exhibited differential expression profiles in different taproot developmental stages among four differently colored radish lines. A number of RsMYBs were highly expressed in the pigmented root tissues at the maturity stage, several genes including RsMYB41, RsMYB117, and RsMYB132 being homologous to PAP1/2, showed high expression levels in the red skin of NAU-YH (red skin-white flesh) taproot, while RsMYB65 and RsMYB159 were highly expressed in the purple root skin of NAU-YZH (purple skin-red flesh), indicating that these RsMYBs might positively regulate the process of anthocyanin accumulation in radish taproot. These results would provide valuable information for further functional characterization of RsMYBs, and facilitate clarifying the molecular mechanism underlying anthocyanin biosynthesis in radish.

萝卜（芸苔属萝卜）是十字花科的一种重要根部蔬菜作物，其色素根组织中的花青素含量很高。据报道，MYB转录因子（TFs）在植物发育和花色苷代谢中起着至关重要的作用，而PAP1 / 2可以促进花色苷生物合成基因的表达。在这项研究中，在萝卜基因组中共鉴定了187个萝卜MYB基因（RsMYBs），并聚集成32个亚科。其中，159个RsMYB位于9个萝卜染色体上。有趣的是，有14个RsMYB在四个不同颜色的萝卜品系中，在不同的主根发育阶段表现出差异表达谱。若干RsMYBs在着色根组织在成熟期，几个基因，包括高表达RsMYB41，RsMYB117，和RsMYB132是同源PAP1 / 2，显示出在NAU-YH的红色皮肤的高表达水平（红色皮肤白肉）的主根，而RsMYB65和RsMYB159在NAU-YZH的紫色根皮（紫红色的皮肉）中高表达，表明这些RsMYB可能会积极调节萝卜主根中花色苷的积累过程。这些结果将为进一步鉴定RsMYBs提供有价值的信息，并有助于阐明萝卜中花色苷生物合成的分子机制。