Grape hyacinths (Muscari spp.) are popular spring flowering bulbs in Europe and also in Hungary. In the spring of 2017, we came across grape hyacinth plants showing mosaic symptoms, which indicated viral infection. Currently Hyacinth mosaic virus (genus Potyvirus), a proposed member of the genus named Muscari mosaic virus, Arabis mosaic virus (genus Nepovirus), Cucumber mosaic virus (genus Cucumovirus) and Tobacco rattle virus (genus Tobravirus) are known to infect grape hyacinth. Leaf samples of symptomatic grape hyacinths were observed, collected and the presence of potyviruses was proved with potyvirus specific monoclonal antibody by ELISA and by potyvirus specific RT-PCR. Laboratory host plants and seed grown Muscari plants were inoculated with leaf sap and symptoms were recorded. Nucleotide sequences of the cloned fragments were compared to GenBank data. In the case of Muscari ‘Helena’ the highest nucleotide identity in the coat protein coding region was found with two Muscari mosaic virus isolates (95.51% and 95.79%). In the case of the clones derived from Muscari ‘Pink Sunrise’ plant, the highest identity was recorded with Muscari mosaic virus isolates (57.65% and 57.80%) and with a Tulip breaking virus strain (55.13%) indicating the existence of a novel potyvirus species, tentatively named Muscari chlorotic mottle virus. The coat protein sequences were aligned and Maximum Likelihood trees were built to analyze phylogenetic relationships.

葡萄风信子（Muscari spp。）是欧洲和匈牙利流行的春季开花球茎。在2017年春季，我们遇到了葡萄风信子植物，这些植物显示出花叶病症状，表明病毒感染。目前风信子花叶病毒（属马铃薯Y病毒），命名为葡萄花叶病毒属的成员提出的南芥菜花叶病毒（属Nepovirus），黄瓜花叶病毒（属Cucumovirus）和烟草脆裂病毒（属烟草脆裂病毒组已知会感染葡萄风信子。观察，收集有症状葡萄风信子的叶片样品，并通过ELISA和马铃薯病毒特异性RT-PCR用马铃薯病毒特异性单克隆抗体证实了马铃薯病毒的存在。用叶汁接种实验室寄主植物和种子生长的穆斯卡里植物，并记录症状。将克隆的片段的核苷酸序列与GenBank数据进行比较。在穆斯卡里“海伦娜”的情况下，在外壳蛋白编码区中发现了两个分离的穆斯卡里花叶病毒分离株的最高核苷酸同一性（95.51％和95.79％）。如果是穆斯卡里的克隆``粉红色日出''植物中，与穆斯卡里花叶病毒分离株（57.65％和57.80％）和郁金香裂伤病毒株（55.13％）的最高身份相同，表明存在一种新型马铃薯病毒，暂定名为穆斯卡里绿斑驳斑病毒。对外壳蛋白序列进行比对，并建立最大似然树以分析系统发生关系。