Thysanolaena maxima (Roxb.) Kuntze, a perennial grass plant, is usually distributed in hilly regions of the Indian Subcontinent. Different parts of T. maxima have been used as herbal medicine by traditional healers of this region. In this present study, T. maxima plant extract has been screened for examination of its secondary metabolite content with their probable cytotoxic activity. Secondary metabolites of the crude T. maxima plant extract were isolated by different chromatographic methods. The structures were elucidated by spectroscopic data (1H NMR, 13C NMR) as well as comparison with available literature sources. Antiradical activity by DPPH radical scavenging assay and antimicrobial activity by disc diffusion method of the fractions and cytotoxic activity by trypan blue exclusion method of the isolated compounds were also evaluated. Three phenolic compounds 4-hydroxybenzaldehyde (1), 4-hydroxycinnamic acid (2), 4-hydroxybenzoic acid (3) and two steroids stigmast-4-en-3-one (4) and β-stigmasterol (5) were isolated from the aerial part of T. maxima. Among the compounds 4-hydroxycinnamic acid, 4-hydroxybenzoic acid and stigmast-4-en-3-one exhibited notable cytotoxic activity against African Green Monkey Kidney Cell line (Vero cell). Bioassay investigation of the isolated compounds and fractions suggested that T. maxima could be a potential source of bioactive secondary metabolites.

中文翻译：

---

最大值的Thysanolaena Roxb的细胞毒性代谢产物。在孟加拉国可用

百里香百里香（Thysanolaena maxima（Roxb。）Kuntze）是多年生草科植物，通常分布在印度次大陆的丘陵地区。该地区的传统治疗师已将巨大锥虫的不同部位用作草药。在本研究中，已经筛选了最大的T. plant植物提取物，以检查其次生代谢产物的含量及其可能的细胞毒性活性。通过不同的色谱方法分离了粗麻黄植物提取物的次生代谢产物。通过光谱数据（1 H NMR，13 C NMR）以及与可用文献来源的比较阐明了结构。还评估了通过DPPH自由基清除法测定的抗自由基活性和通过碟片扩散法测定级分的抗微生物活性，并通过台盼蓝排除法对分离出的化合物的细胞毒活性进行了评估。从中分离出三种酚类化合物4-羟基苯甲醛（1），4-羟基肉桂酸（2），4-羟基苯甲酸（3）和两种甾族化合物stigmast-4-en-3-one（4）和β-豆甾醇（5）。 T. maxima的空中部分。在化合物4-羟基肉桂酸，4-羟基苯甲酸和stigmast-4-en-3-one中，对非洲绿猴肾脏细胞系（Vero细胞）表现出明显的细胞毒性。对分离出的化合物和馏分进行的生物测定研究表明，巨大锥状杆菌可能是生物活性次生代谢产物的潜在来源。4-羟基苯甲酸和stigmast-4-en-3-one对非洲绿猴肾脏细胞系（Vero细胞）表现出明显的细胞毒活性。对分离出的化合物和馏分进行的生物测定研究表明，巨大锥状杆菌可能是生物活性次生代谢产物的潜在来源。4-羟基苯甲酸和stigmast-4-en-3-one对非洲绿猴肾脏细胞系（Vero细胞）表现出明显的细胞毒活性。对分离出的化合物和馏分进行的生物测定研究表明，最大的三角洲可能是生物活性次生代谢产物的潜在来源。