Pectin has recently attracted increasing attention for biomedical and pharmaceutical applications. Due to the lack of adhesion molecules in polysaccharides, phenolic hydroxyl conjugated gelatin was added to enzymatically-gellable peroxidase-modified pectin derivative and compared with phenolic hydroxyl -pectin/collagen. Both pectin and gelatin were modified by tyramine hydrochloride in the presence of EDC/NHS. The phenolic hydroxyl -pectin/phenolic hydroxyl -gelatin, phenolic hydroxyl-pectin/collagen, and phenolic hydroxyl -pectin hydrogels were prepared using horseradish peroxidase and hydrogen peroxide_. The hydrogels were characterized by gelation time analysis. Morphology, enzymatic biodegradation, mechanical and swelling properties as well as water vapor transmission rate were also evaluated. Fibroblasts were cultured for 7 days, and the survival rate was evaluated using conventional MTT assay. Hydrogels composed of Ph-pectin/Ph-gelatin showed decreased biodegradation rate, and WVTR and further improved mechanical performance in comparison with other groups. Both phenolic hydroxyl -pectin/collagen and phenolic hydroxyl -pectin/phenolic hydroxyl -gelatin hydrogels exhibited porous structures. The hydrogels composed of collagen promoted cell survival rate 1.4 and 3.5 times compared to phenolic hydroxyl -gelatin and phenolic hydroxyl -pectin based hydrogels at the end of 7 days, respectively (p < 0.001). The study demonstrated the potential of enzymatically-gellable pectin-based hydrogels as cost-effective frameworks for use in tissue engineering applications.

果胶最近在生物医学和制药应用中引起了越来越多的关注。由于多糖中缺乏粘附分子，将酚羟基共轭明胶添加到酶可凝胶化过氧化物酶修饰的果胶衍生物中，并与酚羟基-果胶/胶原蛋白进行比较。在 EDC/NHS 存在下，用盐酸酪胺对果胶和明胶进行改性。使用辣根过氧化物酶和过氧化氢制备酚羟基果胶/酚羟基明胶、酚羟基果胶/胶原蛋白和酚羟基果胶水凝胶_。通过凝胶时间分析表征水凝胶。还评估了形态、酶促生物降解、机械和溶胀性能以及水蒸气透过率。成纤维细胞培养7天，并使用常规MTT测定法评估存活率。与其他组相比，由 Ph-果胶/Ph-明胶组成的水凝胶显示出生物降解率降低、WVTR 和机械性能进一步提高。酚羟基果胶/胶原蛋白和酚羟基果胶/酚羟基明胶水凝胶均表现出多孔结构。在 7 天结束时，与基于酚羟基明胶和酚羟基果胶的水凝胶相比，由胶原组成的水凝胶分别将细胞存活率提高了 1.4 倍和 3.5 倍（p < 0.001）。