Abstract

Colorectal cancer is an important concern in modern society. Risk factors such as the diet indicate the need to find healthy food products displaying additional health benefits. This study aimed to characterise and evaluate the impact of the colonic metabolites from the fermented non-digestible fraction of Moringa oleifera (MO) leaves (FNFM) on cell death mechanisms from HT-29 cells. MO leaves were digested in vitro, and the 12 h-colonic extract was obtained. FNFM mainly contained morin and chlorogenic acids (41.97 and 25.33 µg/g sample). Butyric acid was ranked as the most important metabolite of FNFM. The FNFM exerted antiproliferative effect against HT-29 colorectal cancer cells (half lethal concentration, LC₅₀: 5.9 mL/100 mL). Compared to untreated control, LC₅₀ increased H2O2 production (149.43%); induced apoptosis (119.02%), autophagy (75.60%), and necrosis (87.72%). These results suggested that digested MO colonic metabolites exert antiproliferative effect against HT-29 cells, providing additional health benefits associated with MO consumption.

摘要

大肠癌是现代社会中的重要问题。饮食等风险因素表明，需要寻找表现出更多健康益处的健康食品。这项研究旨在表征和评估来自辣木（MO）叶（FNFM）发酵的不可消化部分的结肠代谢产物对HT-29细胞死亡机制的影响。MO叶片在体外被消化，并获得了12小时的结肠提取物。FNFM主要包含味精和绿原酸（41.97和25.33 µg / g样品）。丁酸被评为FNFM最重要的代谢产物。FNFM对HT-29大肠癌细胞具有抗增殖作用（半致死浓度，LC ₅₀：5.9 mL / 100 mL）。与未处理的对照组相比，LC ₅₀增加了H2O2的产生（149.43％）；诱导细胞凋亡（119.02％），自噬（75.60％）和坏死（87.72％）。这些结果表明，消化的MO结肠代谢产物对HT-29细胞具有抗增殖作用，从而提供与MO摄入相关的其他健康益处。