ABSTRACT

Aim

This study aimed to evaluate the protective effect of flaxseed mucilage on the co-extrusion microencapsulation of Lactobacillus rhamnosus GG.

Methods

Core flow rate, chitosan coating, and flaxseed mucilage concentration were optimized for the microencapsulation of L. rhamnosus. The microbeads were characterized and evaluated on microencapsulation efficiency and cell released after 6 h of sequential digestion.

Results

The optimized parameters for the L. rhamnosus microencapsulation were 1.0 mL/min core flow rate, 0.4% (w/v) chitosan coating and 0.4% (w/v) flaxseed mucilage. The L. rhamnosus microbeads with flaxseed mucilage in core and wall materials had a smooth surface with 781.3 µm diameter, the highest microencapsulation efficiency (98.8% w/w), lowest swelling (5196.7% w/w) and erosion ratio (515.5% w/w), and least cell release (< 40% w/w) with 9.31 log₁₀ CFU mL⁻¹ after sequential digestion.

Conclusions

This study showed the protective capacity of flaxseed mucilage towards the L. rhamnosus GG during microencapsulation and gastrointestinal environment.

中文翻译：

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共挤技术将鼠李糖乳杆菌与亚麻籽微胶微囊化

摘要

目标

这项研究旨在评估亚麻粘液对鼠李糖乳杆菌GG共挤出微囊化的保护作用。

方法

优化了鼠李糖乳杆菌微囊化的核心流速，壳聚糖涂层和亚麻籽粘液浓度。对微珠进行表征并评估其微囊化效率，并在连续消化6小时后释放细胞。

结果

鼠李糖乳杆菌微囊化的优化参数为1.0 mL / min核心流速，0.4％（w / v）的脱乙酰壳多糖涂层和0.4％（w / v）的亚麻籽粘液。的鼠李糖乳杆菌与芯和墙壁材料亚麻籽粘液微珠具有光滑的表面具有781.3微米的直径，所述微胶囊化最高效率（98.8％重量/重量），肿胀最低（5196.7％重量/重量）和侵蚀率（515.5％（重量） / w），并在连续消化后以9.31 log ₁₀ CFU mL ^-1释放最少的细胞（<40％w / w）。

结论

这项研究显示了在微囊化和胃肠道环境中，亚麻籽粘液对鼠李糖乳杆菌的保护能力。