Black pepper (Piper nigrum L.; 2n= 52; Piperaceae), the king of spices, is a perennial, trailing woody flowering vine and has global importance with widespread dietary, medicinal, and preservative uses. It is an economically-important germplasm cultivated for its fruit and the major cash crop in >30 tropical countries. Crop production is mainly affected by drought stress. The present study deals with the candidate gene identification from drought-affected black pepper leaf transcriptome generated by Illumina Hiseq2000. It also aims to mine putative molecular markers (namely SSRs, SNPs and InDels) and generate primers for them. The identification of transcription factors and pathways involved in drought tolerance is also reported here. De novo transcriptome assembly was performed with trinity assembler. In total, 4914 differential expressed genes, 2110 transcriptional factors, 786 domains and 1137 families, 20124 putative SSR markers and 259236 variants were identified. At2g30105 (unidentified gene containing leucine-rich repeats and ubiquitin-like domain), serine threonine protein kinase, Mitogen-activated protein kinase, NBS-LRR, Myeloblastosis related proteins, Basic helix-loop-helix are all found upregulated and are reported to be associated with plant tolerance against drought condition. All these information are catalogued in the Black Pepper Drought Transcriptome Database (BPDRTDb), freely accessible for academic use at http://webtom.cabgrid.res.in/bpdrtdb/. This database is a good foundation for the genetic improvement of pepper plants, breeding programmes and mapping population of this crop. Putative markers can also be a reliable genomic resource to develop drought-tolerant variety for better black pepper productivity. This article is protected by copyright. All rights reserved.

中文翻译：

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黑胡椒（Piper nigrum L.）的干旱响应性：相关基因和网络基因组资源的开发

黑胡椒 (Piper nigrum L.; 2n= 52; Piperaceae) 是香料之王，是一种多年生、尾随木本开花的藤本植物，具有广泛的饮食、药用和防腐剂用途，具有全球重要性。它是一种重要的经济种质，在 30 多个热带国家种植其果实和主要经济作物。作物生产主要受干旱胁迫的影响。本研究涉及从 Illumina Hiseq2000 生成的受干旱影响的黑胡椒叶转录组中鉴定候选基因。它还旨在挖掘假定的分子标记（即 SSR、SNP 和 InDels）并为它们生成引物。此处还报告了与耐旱性有关的转录因子和途径的鉴定。使用三位一体组装器进行从头转录组组装。总共，鉴定了4914个差异表达基因、2110个转录因子、786个结构域和1137个家族、20124个假定的SSR标记和259236个变异。At2g30105（含有富含亮氨酸重复序列和泛素样域的未鉴定基因）、丝氨酸苏氨酸蛋白激酶、丝裂原活化蛋白激酶、NBS-LRR、成髓细胞相关蛋白、Basic helix-loop-helix 都被发现上调，据报道与植物对干旱条件的耐受性有关。所有这些信息都编目在黑胡椒干旱转录组数据库 (BPDRTDb) 中，可在 http://webtom.cabgrid.res.in/bpdrtdb/ 免费访问供学术使用。该数据库为辣椒植物的遗传改良、育种计划和该作物的种群定位奠定了良好的基础。推定的标记也可以是可靠的基因组资源，用于开发耐旱品种以提高黑胡椒产量。本文受版权保护。版权所有。