Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest,Insect Molecular Biology

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Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest
Insect Molecular Biology ( IF 2.3 ) Pub Date : 2020-12-11 , DOI: 10.1111/imb.12688
K B Ferguson ₁ , S Visser _{2,

3} , M Dalíková _{2,

3} , I Provazníková _{2,

3,

4} , A Urbaneja ₅ , M Pérez-Hedo ₅ , F Marec ₂ , J H Werren ₆ , B J Zwaan ₁ , B A Pannebakker ₁ , E C Verhulst ₇

Affiliation

Nesidiocoris tenuis (Reuter) is an efficient predatory biological control agent used throughout the Mediterranean Basin in tomato crops but regarded as a pest in northern European countries. From the family Miridae, it is an economically important insect yet very little is known in terms of genetic information and no genomic or transcriptomic studies have been published. Here, we use a linked‐read sequencing strategy on a single female N. tenuis. From this, we assembled the 355 Mbp genome and delivered an ab initio, homology‐based and evidence‐based annotation. Along the way, the bacterial “contamination” was removed from the assembly. In addition, bacterial lateral gene transfer (LGT) candidates were detected in the N. tenuis genome. The complete gene set is composed of 24 688 genes; the associated proteins were compared to other hemipterans (Cimex lectularis, Halyomorpha halys and Acyrthosiphon pisum). We visualized the genome using various cytogenetic techniques, such as karyotyping, CGH and GISH, indicating a karyotype of 2n = 32. Additional analyses include the localization of 18S rDNA and unique satellite probes as well as pooled sequencing to assess nucleotide diversity and neutrality of the commercial population. This is one of the first mirid genomes to be released and the first of a mirid biological control agent.

中文翻译：

杰基尔还是海德？Nesidiocoris tenuis 的基因组（及更多），一种食草性捕食性昆虫，既是生物防治剂又是害虫

Nesidiocoris tenuis (路透社) 是一种高效的捕食性生物防治剂，在整个地中海盆地的番茄作物中使用，但在北欧国家被视为害虫。它属于蝽科，是一种重要的经济昆虫，但人们对遗传信息知之甚少，也没有发表基因组或转录组学研究。在这里，我们对单个雌性细细猪笼草使用了链接读取测序策略。由此，我们组装了 355 Mbp 基因组，并提供了从头开始、基于同源性和基于证据的注释。在此过程中，细菌“污染”被从组件中去除。此外，在N. tenuis基因组中检测到细菌横向基因转移（LGT）候选者。完整的基因集由24 688个基因组成；将相关蛋白与其他半翅目动物（Cimex lectularis、Halyomorpha halys和Acyrthosiphon pisum）进行了比较。我们使用各种细胞遗传学技术（例如核型分析、CGH 和 GISH）对基因组进行可视化，表明核型为 2 n = 32。其他分析包括 18S rDNA 的定位和独特的卫星探针以及用于评估核苷酸多样性和中性的汇总测序。商业人口。这是首批发布的Mirid 基因组之一，也是第一个Mirid 生物防治剂。

更新日期：2020-12-11

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