Bombyx mori cypovirus 1 (BmCPV1) is a member of the Reoviridae family which is characterized by its single-layered capsid. Similar with other turreted viruses in the Reoviridae, transcription of BmCPV1 occurs inside the capsid, and the nascent mRNA is released to the turret which consists of five turret proteins (TPs) and located at the 5-fold axis of the outer capsid, then the capping enzyme TP will guanylate and methylate the nascent viral mRNA to produce a matured mRNA. However, during these processes, how the BmCPV1 draws other cellular proteins to facilitate its replication is still lesser-known. Here we used an ELISA to investigate the interaction between ALP and BmCPV1. A co-immunoprecipitation technique was employed to detect the interaction of ALP with the Methylase domain of TP. We further studied whether ALP affects the replication of BmCPV1 inside the cell, results show that reducing the expression of ALP through RNAi reduced the transcription level of the BmCPV1 VP1 gene, which was increased by overexpression of ALP. In summary, our data demonstrate an interaction between ALP and BmCPV1 and that ALP promoted the replication of BmCPV1, and support our hypothesis of the ALP is an RTPase to facilitate the capping process of BmCPV1.

家蚕cypovirus 1 (BmCPV1) 是呼肠孤病毒科的成员，其特征是其单层衣壳。与呼肠孤病毒科中的其他转塔病毒相似, BmCPV1 的转录发生在衣壳内部，新生的 mRNA 被释放到由五个转塔蛋白 (TPs) 组成的转塔，位于外衣壳的 5 倍轴，然后加帽酶 TP 将鸟苷酸化和甲基化新生病毒 mRNA 以产生成熟的 mRNA。然而，在这些过程中，BmCPV1 如何吸引其他细胞蛋白以促进其复制仍然鲜为人知。在这里，我们使用 ELISA 来研究 ALP 和 BmCPV1 之间的相互作用。采用共免疫沉淀技术检测 ALP 与 TP 甲基化酶结构域的相互作用。我们进一步研究了 ALP 是否影响 BmCPV1 在细胞内的复制，结果表明通过 RNAi 降低 ALP 的表达降低了 BmCPV1 VP1的转录水平ALP 的过度表达增加了基因。总之，我们的数据表明 ALP 和 BmCPV1 之间存在相互作用，并且 ALP 促进了 BmCPV1 的复制，并支持我们的假设，即 ALP 是一种促进 BmCPV1 加帽过程的 RTPase。