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Sample pooling strategies for SARS-CoV-2 detection
Journal of Virological Methods ( IF 2.2 ) Pub Date : 2020-12-11 , DOI: 10.1016/j.jviromet.2020.114044
Nefeli Lagopati 1 , Panagiota Tsioli 1 , Ioanna Mourkioti 1 , Aikaterini Polyzou 1 , Angelos Papaspyropoulos 1 , Alexandros Zafiropoulos 2 , Konstantinos Evangelou 1 , George Sourvinos 2 , Vassilis G Gorgoulis 3
Affiliation  

The worldwide COVID-19 pandemic outburst has caused a serious public health issue with increasing needs of accurate and rapid diagnostic and screening testing. This situation requires an optimized management of the chemical reagents, the consumables, and the human resources, in order to respond accurately and effectively, controlling the spread of the disease. Testing on pooled samples maximizes the number of tested samples, by minimizing the time and the lab supplies needed. The general conceptualization of the pooling method is based on mixing samples together in a batch. Individual testing is needed only if a specific pool exhibits a positive result. The development of alternative hybrid methods, based on “in house” protocols, utilizing commercially available consumables, in combination with a reliable pooling method would provide a solution, focusing on the better exploitation of the personnel and the lab supplies, allowing for rapid screening of a population in a reasonably short time.



中文翻译:

用于 SARS-CoV-2 检测的样本合并策略

全球爆发的 COVID-19 大流行引发了严重的公共卫生问题,对准确快速的诊断和筛查检测的需求日益增加。这种情况需要对化学试剂、耗材和人力资源进行优化管理,以便准确有效地做出反应,控制疾病的传播。通过最大限度地减少所需的时间和实验室用品,对混合样本进行测试可以最大限度地增加测试样本的数量。池化方法的一般概念化是基于将样本批量混合在一起。仅当特定池显示阳性结果时才需要进行单独测试。替代混合方法的开发,基于“内部”协议,利用市售耗材,结合可靠的汇集方法,将提供一种解决方案,

更新日期:2020-12-26
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