In this study, high-performance liquid chromatography (HPLC) and colorimeter were applied to evaluate the quality of different species and differently prepared slices of Zedoray Rhizome samples with the aid of chemometric tools. Fifty batches of Zedoray Rhizome samples from different species and forty-two batches of Zedoray Rhizome samples from differently prepared slices were collected. The quantitative method was developed using HPLC to simultaneously determine the contents of twelve chemical ingredients in Zedoray Rhizome. The colour parameters L, a, and b were measured by a colorimeter. Then, the collected data were analyzed by the principal component analysis and Pearson correlation analysis. The results showed that the proposed method was capable of accurately determining the contents of the twelve chemical ingredients and the colour parameters for the collected samples. There was a dramatic difference in the contents of the chemical ingredients and in the colour parameters among different species and differently prepared slices of Zedoray Rhizome samples. This study reveals that combining HPLC, colorimeter, and chemometric tools can provide a new approach to comprehensively evaluate the quality of Zedoray Rhizome samples.

中文翻译：

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高效液相色谱和色度计在化学计量学的帮助下对不同种类和不同制备的莪术根切片的质量评估

在这项研究中，借助化学计量工具，使用高效液相色谱（HPLC）和色度计来评估不同物种和不同制备的Zedoray根茎样品切片的质量。收集了来自不同物种的五十批次的根瘤菌根样品和来自不同制备的切片的四十二批次的根瘤菌根样品。使用HPLC建立了定量方法，以同时测定Zedoray根茎中12种化学成分的含量。通过色度计测量颜色参数L，a和b。然后，通过主成分分析和皮尔逊相关分析对收集的数据进行分析。结果表明，该方法能够准确测定所采集样品中十二种化学成分的含量和颜色参数。在不同物种和不同制备的Zedoray根茎样品切片之间，化学成分含量和颜色参数存在巨大差异。这项研究表明，结合HPLC，比色计和化学计量学工具可以提供一种全面评估Zedoray根茎样品质量的新方法。