We report on polyethylenimine (PEI)-mediated synthesis of Prussian blue nanoparticles (PBNPs) and gold nanoparticles (AuNPs); the formation of PBNP-AuNP nanohybrids with a remarkable change in Prussian blue character as a function of gold cation concentration was also considered. It was shown that PEI-protected polycrystalline PBNPs can be synthesized in an acidic medium from the precursor potassium ferricyanide [K₃Fe(CN)₆] at 60 °C. Since PEI also enables the controlled formation of gold nanoparticles (AuNPs) in the presence of formaldehyde under ambient conditions, nanohybrids of PBNPs and AuNPs were prepared. The formation of AuNPs was recorded over a wide range of PEI concentrations, which allowed control over polymeric cation capping of the AuNPs. PEI concentration-dependent enhancement/quenching of fluorescence/resonance Rayleigh scattering was useful for non-enzymatic detection of serum glucose levels. The resonance Rayleigh scattering intensity of PBNPs was several-fold higher than that of AuNPs and acted as a potent quencher of fluorescence. At an optimal concentration of PEI, AuNPs allowed an increase in the fluorescence signal as function of glucose concentration; the quenching ability of PB was demonstrated to be a function of the glucose concentration. This method is efficient for fast glucose sensing and offers a wider linear dynamic range, 0–10 mM, which is useful for non-enzymatic detection of serum glucose levels.

我们报告了聚乙烯亚胺 (PEI) 介导的普鲁士蓝纳米粒子 (PBNPs) 和金纳米粒子 (AuNPs) 的合成；还考虑了 PBNP-AuNP 纳米杂化物的形成，其普鲁士蓝特性随金阳离子浓度的变化而显着变化。结果表明，PEI 保护的多晶 PBNPs 可以在酸性介质中由前体铁氰化钾 [K ₃ Fe(CN) _6合成] 在 60 °C。由于 PEI 还能够在环境条件下在甲醛存在的情况下控制金纳米粒子 (AuNPs) 的形成，因此制备了 PBNPs 和 AuNPs 的纳米杂化物。在很宽的 PEI 浓度范围内记录了 AuNPs 的形成，这允许控制 AuNPs 的聚合阳离子封端。荧光/共振瑞利散射的 PEI 浓度依赖性增强/猝灭可用于血清葡萄糖水平的非酶检测。PBNPs 的共振瑞利散射强度比 AuNPs 高几倍，并作为有效的荧光猝灭剂。在 PEI 的最佳浓度下，AuNPs 允许作为葡萄糖浓度函数的荧光信号增加；PB的淬灭能力被证明是葡萄糖浓度的函数。