As a hybrid optical microscopic imaging technology, photoacoustic microscopy images the optical absorption contrasts and takes advantage of low acoustic scattering of biological tissues to achieve high-resolution anatomical and functional imaging. When combined with other imaging modalities, photoacoustic microscopy-based multimodal technologies can provide complementary contrast mechanisms to reveal complementary information of biological tissues. To achieve intrinsically and precisely registered images in a multimodal photoacoustic microscopy imaging system, either the ultrasonic transducer or the light source can be shared among the different imaging modalities. These technologies are the major focus of this minireview. It also covered the progress of the recently developed penta-modal photoacoustic microscopy imaging system featuring a novel dynamic focusing technique enabled by OCT contour scan.

作为一种混合光学显微成像技术，光声显微技术通过对光吸收对比进行成像，并利用生物组织的低声散射来实现高分辨率的解剖和功能成像。当与其他成像模式相结合时，基于光声显微镜的多模式技术可以提供互补的对比机制来揭示生物组织的互补信息。为了在多模态光声显微镜成像系统中获得内在和精确配准的图像，超声换能器或光源可以在不同的成像模式之间共享。这些技术是本次小评的主要焦点。