ABSTRACT Hyperspectral reflectance is becoming an increasingly popular source of information for plants’ physio-chemical status. In this study, net photosynthetic rate (A) and the activity of four antioxidant enzymes of Phaseolus vulgaris L., superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX), were compared with hyperspectral data using regression methods (principal component regression (PCR) and partial least squares regression (PLSR)) and vegetation indices (normalized difference vegetation index (NDVI), photochemical reflectance index (PRI), water index (WI) and the normalized reflectance (R) ratio R 710/R 750). The experiment was conducted on potted plants of two common bean genotypes (‘Blue lake’ and ‘Terli’), receiving four treatments (control, drought, salinity and drought + salinity), in an unheated greenhouse. The results suggest that both types of abiotic stress have a significant impact on the photosynthetic operation and the function of the antioxidant mechanism. The two regression methods performed well in predicting photosynthetic rate (A), while a strong correlation between A with the two vegetation indices was also revealed. Antioxidant enzymes appeared to be correlated with the WI (GPX and SOD enzymes) and the normalized reflectance (R) ratio R 710/R 750 (APX and SOD enzymes). Hyperspectral data seem to be particularly useful for fast and non-destructive assessment method for photosynthesis and enzymes activity of common bean plant.

中文翻译：

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多重胁迫下两种菜豆基因型生理生化测量值与光谱反射率的关系

摘要 高光谱反射正成为植物理化状态信息的日益流行的来源。在本研究中，将菜豆的净光合速率 (A) 和四种抗氧化酶超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、抗坏血酸过氧化物酶 (APX) 和愈创木酚过氧化物酶 (GPX) 的活性与高光谱进行了比较。数据使用回归方法（主成分回归（PCR）和偏最小二乘回归（PLSR））和植被指数（归一化差异植被指数（NDVI）、光化学反射指数（PRI）、水指数（WI）和归一化反射率（R） ) 比率 R 710/R 750)。该实验在两种常见豆类基因型（'蓝湖'和'特利'）的盆栽植物上进行，接受四个处理（对照、干旱、盐度和干旱+盐度），在未加热的温室中。结果表明，两种类型的非生物胁迫对光合运行和抗氧化机制的功能都有显着影响。这两种回归方法在预测光合速率 (A) 方面表现良好，同时还揭示了 A 与两个植被指数之间的强相关性。抗氧化酶似乎与 WI（GPX 和 SOD 酶）和归一化反射率 (R) 比 R 710/R 750（APX 和 SOD 酶）相关。高光谱数据似乎对普通豆类植物的光合作用和酶活性的快速无损评估方法特别有用。这两种回归方法在预测光合速率 (A) 方面表现良好，同时还揭示了 A 与两个植被指数之间的强相关性。抗氧化酶似乎与 WI（GPX 和 SOD 酶）和归一化反射率 (R) 比 R 710/R 750（APX 和 SOD 酶）相关。高光谱数据似乎对普通豆类植物的光合作用和酶活性的快速无损评估方法特别有用。这两种回归方法在预测光合速率 (A) 方面表现良好，同时还揭示了 A 与两个植被指数之间的强相关性。抗氧化酶似乎与 WI（GPX 和 SOD 酶）和归一化反射率 (R) 比 R 710/R 750（APX 和 SOD 酶）相关。高光谱数据似乎对普通豆类植物的光合作用和酶活性的快速无损评估方法特别有用。