ABSTRACT

We conducted a cytological search for amyloid structures in female reproductive cells of Gallus gallus domesticus and Drosophila melanogaster. We have shown that the amyloid-specific dye, Thioflavin S, but not Congo red, stains some cytoplasmic and even nuclear structures in chicken ovaries. In fruit fly eggs both Thioflavin S and Congo red specifically stain eggshell structures such as micropyle, dorsal appendages and pillars. Moreover, these structures, when stained with Congo red, demonstrate birefringence in polarized light, which is a characteristic feature of all classical amyloids. Our data show that female reproductive cells during evolution began to use amyloid fibrils to form various functional structures necessary for development under certain environmental conditions.

摘要

我们进行了细胞学搜索家鸡和黑果蝇的女性生殖细胞中的淀粉样结构。我们已经证明，淀粉样蛋白特异性染料硫黄素S而非刚果红在鸡卵巢中染色了一些细胞质甚至核结构。在果蝇卵中，硫黄素S和刚果红均会特异性地污染蛋壳结构，例如小孔，背附肢和柱子。此外，这些结构用刚果红染色时，在偏振光中表现出双折射，这是所有经典淀粉样蛋白的特征。我们的数据表明，雌性生殖细胞在进化过程中开始使用淀粉样蛋白原纤维来形成在某些环境条件下发育所必需的各种功能结构。