Journal of Oral Microbiology ( IF 3.7 ) Pub Date : 2020-12-09 Paulo H. Braz-Silva, Ana C. Mamana, Camila M. Romano, Alvina C. Felix, Anderson V. de Paula, Noeli E Fereira, Lewis F. Buss, Tania R. Tozetto-Mendoza, Rafael A. V. Caixeta, Fabio E. Leal, Regina M. Z. Grespan, João C. S. Bizário, Andrea B. C. Ferraz, Dipak Sapkota, Simone Giannecchini, Kelvin K. To, Alain Doglio, Maria C. Mendes-Correa
ABSTRACT
Background: SARS-CoV-2 quickly spreads in the worldwide population, imposing social restrictions to control the infection, being the massive testing another essential strategy to break the chain of transmission.
Aim: To compare the performance of at-home self-collected samples – saliva and combined nasal-oropharyngeal swabs (NOP) – for SARS-CoV-2 detection in a telemedicine platform for COVID-19 surveillance.
Material and methods: We analyzed 201 patients who met the criteria of suspected COVID-19. NOP sampling was combined (nostrils and oropharynx) and saliva collected using a cotton pad device. Detection of SARS-COV-2 was performed by using the Altona RealStar® SARS-CoV-2 RT-PCR Kit 1.0.
Results: There was an overall significant agreement (κ coefficient value of 0.58) between saliva and NOP. Considering results in either sample, 70 patients positive for SARS-CoV-2 were identified, with 52/70 being positive in NOP and 55/70 in saliva. This corresponds to sensitivities of 74.2% (95% CI; 63.7% to 83.1%) for NOP and 78.6% (95% CI; 67.6% to 86.6%) for saliva.
Conclusion: Our data show the feasibility of using at-home self-collected samples (especially saliva), as an adequate alternative for SARS-CoV-2 detection. This new approach of testing can be useful to develop strategies for COVID-19 surveillance and for guiding public health decisions.
中文翻译:
在家自行收集的唾液和鼻咽咽拭子在监测COVID-19中的表现
摘要
背景:SARS-CoV-2在全球人口中迅速传播,强加了控制感染的社会限制,这是对打破传播链的另一项重要策略的大规模测试。
目的:比较在家自我收集的样本(唾液和鼻咽拭子组合(NOP))在用于COVID-19监视的远程医疗平台中检测SARS-CoV-2的性能。
材料和方法:我们分析了201名符合可疑COVID-19标准的患者。合并NOP采样(鼻孔和口咽),并使用棉垫装置收集唾液。SARS-COV-2的检测是通过使用AltonaRealStar®SARS-CoV-2 RT-PCR Kit 1.0进行的。
结果:唾液和NOP之间存在总体显着一致性(κ系数值为0.58)。考虑到两个样本的结果,确定了70例SARS-CoV-2阳性患者,其中NOP阳性52/70,唾液中55/70。这对应于NOP的敏感性为74.2%(95%CI; 63.7%至83.1%),唾液的敏感性为78.6%(95%CI; 67.6%至86.6%)。
结论:我们的数据表明,使用在家自行收集的样本(尤其是唾液)作为SARS-CoV-2检测的适当替代方法的可行性。这种新的测试方法对于制定COVID-19监视策略和指导公共卫生决策很有用。