Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial‐mesenchymal transition‐related markers,FEBS Open Bio

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Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial‐mesenchymal transition‐related markers
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-12-10 , DOI: 10.1002/2211-5463.13062
Yang Li ₁ , Hui Gong ₂ , Lei Feng ₂ , Dan Mao ₃ , Yujie Xiao ₄ , Yunqi Wang ₁ , Lizhong Huang ₄

Affiliation

Chondroitin polymerizing factor (CHPF) plays an important role in the development of certain malignant tumors. However, the role of CHPF in breast carcinoma (BRCA) and its underlying mechanism are still not fully elucidated. Expression profiles for CHPF in BRCA tissues were retrieved from The Cancer Genome Atlas database and used for prognostic analysis. Cell viability, invasion and migration were measured in vitro using MCF7 and MDA‐MB‐231 cell lines upon knockdown or over‐expression of CHPF. Bioinformatic analysis showed that CHPF was substantially upregulated in BRCA tissues, and a quantitative reverse transcriptase‐PCR was performed to confirm its upregulation in BRCA cells. High expression of CHPF was observed to be significantly associated with pathologic stage, metastasis and worse prognosis. We also observed that depletion of CHPF significantly inhibited cell proliferative, invasive and migratory abilities, whereas overexpression of CHPF exerted the opposite effects. Furthermore, analysis of the GEPIA database revealed that CHPF expression is positively correlated with the epithelial–mesenchymal transition‐related markers vimentin, Snail, Slug and motion‐related protein matrix metallopeptidase 2; these findings were confirmed via western blotting. Our data suggest that CHPF may contribute to BRCA progression by modulating epithelial–mesenchymal transition‐related markers and matrix metallopeptidase 2 expression.

中文翻译：

软骨素聚合因子促进乳腺癌细胞增殖、侵袭和迁移并影响上皮间质转化相关标志物的表达

软骨素聚合因子（CHPF）在某些恶性肿瘤的发生发展中起重要作用。然而，CHPF 在乳腺癌 (BRCA) 中的作用及其潜在机制仍未完全阐明。BRCA 组织中 CHPF 的表达谱从癌症基因组图谱数据库中检索并用于预后分析。在体外测量细胞活力、侵袭和迁移在敲低或过表达 CHPF 后使用 MCF7 和 MDA-MB-231 细胞系。生物信息学分析表明，CHPF 在 BRCA 组织中显着上调，并且进行了定量逆转录酶 PCR 以确认其在 BRCA 细胞中的上调。观察到CHPF的高表达与病理分期、转移和预后不良显着相关。我们还观察到 CHPF 的消耗显着抑制细胞增殖、侵袭和迁移能力，而 CHPF 的过表达则产生相反的效果。此外，对 GEPIA 数据库的分析显示，CHPF 表达与上皮间质转化相关标志物波形蛋白、Snail、Slug 和运动相关蛋白基质金属肽酶 2 呈正相关；这些发现通过蛋白质印迹得到证实。

更新日期：2021-02-11

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