CTP synthase (CTPS) cytoophidia have been found in many species over domains of life in the past 10 years, implying the evolutionary conservation of these structures. However, there are differences in cytoophidia between species. The difference in CTPS cytoophidium properties between budding yeast (Saccharomyces cerevisiae) and fission yeast (Schizosaccharomyces pombe) inspires this research. We study the effects of culture environment on cytoophidia in S. cerevisiae by switching to the optimal medium for S. pombe. S. cerevisiae CTPS cytoophidium fragmentation and pseudohyphae formation are observed after treatment with S. pombe medium YES instead of S. cerevisiae medium YPD. By modifying the level of each ingredient of the media, we find that hypoosmolality impedes cytoophidium integrity during nitrogen starvation. Our study demonstrates the relationship between cytoophidium integrity and environmental stress, supporting the role of cytoophidia in stress resistance.

中文翻译：

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在氮饥饿期间，低渗透压会阻碍 cytoophidium 的完整性

在过去的 10 年中，已经在许多物种的生命领域中发现了 CTP 合酶 (CTPS) cytoophidia，这意味着这些结构的进化保守性。然而，物种之间的嗜细胞菌存在差异。出芽酵母（酿酒酵母）和裂殖酵母（粟酒裂殖酵母）之间 CTPS 细胞毒菌特性的差异激发了这项研究。我们通过切换到S. pombe的最佳培养基来研究培养环境对S. cerevisiae嗜细胞菌的影响。S. cerevisiae CTPS cytoophidium 碎裂和假菌丝形成在用S. pombe培养基 YES 而不是S. cerevisiae处理后观察到中等 YPD。通过修改培养基中每种成分的水平，我们发现低渗透压会在氮饥饿期间阻碍 cytoophidium 的完整性。我们的研究证明了嗜细胞菌完整性与环境应激之间的关系，支持了嗜细胞菌在抗逆性中的作用。