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Structure–function relationship analysis of fucoidan from sea cucumber (Holothuria tubulosa) on ameliorating metabolic inflammation
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-12-09 , DOI: 10.1111/jfbc.13500 Yujie Zhu 1 , Yingying Tian 1 , Na Wang 1 , Yaoguang Chang 1 , Changhu Xue 1 , Jingfeng Wang 1
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-12-09 , DOI: 10.1111/jfbc.13500 Yujie Zhu 1 , Yingying Tian 1 , Na Wang 1 , Yaoguang Chang 1 , Changhu Xue 1 , Jingfeng Wang 1
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The structure–function relationships of sea cucumber fucoidan has been seldom investigated. In this study, the effect of fucoidan which extracted from sea cucumber Holothuria tubulosa (Ht‐FUC) with different molecular weight and chain conformation (Ht1/2/3/4) in metabolic inflammation was investigated. A co‐cultured system comprising adipocytes and macrophages was used to explore the impact in vitro. The high‐fat high‐sucrose diet (HFD)‐fed obese mice model was established to verified the effect of Ht‐FUC in vivo. The results demonstrated that all examined Ht‐FUC attenuated the productions of inflammatory cytokines, promoted the M2 phenotypic polarization of co‐cultured macrophage by activating PPARγ. Furthermore Ht‐FUC impressed lipolysis of the co‐cultured adipocytes by inhibiting TLR4/NF‐κB‐dependent pathway. In vivo, Ht‐FUC especially reduced serum inflammation level, attenuated M1/M2 polarization of liver Kupffer cells, and attenuated inflammatory infiltration of epididymal adipose tissue. Consistently, Ht3 played the best effect, and may be more beneficial for the intervention of metabolic disease.
中文翻译：
海参中岩藻依聚糖对改善代谢炎症的构效关系分析
很少研究海参岩藻依聚糖的结构-功能关系。在这项研究中，从海参Holothuriatubulosa提取的岩藻依聚糖的效果研究了不同分子量和链构象（Ht1 / 2/3/4）在代谢性炎症中的（Ht-FUC）。包含脂肪细胞和巨噬细胞的共培养系统用于探索体外影响。建立了高脂高蔗糖饮食（HFD）喂养的肥胖小鼠模型，以验证Ht-FUC在体内的作用。结果表明，所有检测到的Ht-FUC均通过激活PPARγ减弱了炎性细胞因子的产生，促进了共培养巨噬细胞的M2表型极化。此外，Ht-FUC通过抑制TLR4 /NF-κB依赖性途径而影响共培养的脂肪细胞的脂解作用。在体内，Ht-FUC尤其可降低血清炎症水平，减弱肝库普弗细胞的M1 / M2极化，并减弱附睾脂肪组织的炎症浸润。一致地，
更新日期：2021-01-19
中文翻译：
海参中岩藻依聚糖对改善代谢炎症的构效关系分析
很少研究海参岩藻依聚糖的结构-功能关系。在这项研究中，从海参Holothuriatubulosa提取的岩藻依聚糖的效果研究了不同分子量和链构象（Ht1 / 2/3/4）在代谢性炎症中的（Ht-FUC）。包含脂肪细胞和巨噬细胞的共培养系统用于探索体外影响。建立了高脂高蔗糖饮食（HFD）喂养的肥胖小鼠模型，以验证Ht-FUC在体内的作用。结果表明，所有检测到的Ht-FUC均通过激活PPARγ减弱了炎性细胞因子的产生，促进了共培养巨噬细胞的M2表型极化。此外，Ht-FUC通过抑制TLR4 /NF-κB依赖性途径而影响共培养的脂肪细胞的脂解作用。在体内，Ht-FUC尤其可降低血清炎症水平，减弱肝库普弗细胞的M1 / M2极化，并减弱附睾脂肪组织的炎症浸润。一致地，
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