Intestinal epithelial barrier damage caused by intestinal epithelial cells (IECs) dysfunction plays a crucial role in the pathogenesis and development of inflammatory bowel disease (IBD). Recently, some studies have suggested the emerging role of long non‐coding RNAs (lncRNAs) in IBD. The aim of this study was to reveal lncRNAs and mRNA expression profiles in IECs from a mouse model of colitis and to expand our understanding in the intestinal epithelial barrier regulation. IECs from the colons of wild‐type mice and dextran sulphate sodium (DSS)‐induced mice were isolated for high‐throughput RNA‐sequencing. A total of 254 up‐regulated and 1013 down‐regulated mRNAs and 542 up‐regulated and 766 down‐regulated lncRNAs were detected in the DSS group compared with the Control group. Four mRNAs and six lncRNAs were validated by real‐time quantitative PCR. Function analysis showed that dysregulated mRNAs participated in TLR7 signalling pathway, IL‐1 receptor activity, BMP receptor binding and IL‐17 signalling pathway. Furthermore, the possibility of indirect interactions between differentially expressed mRNAs and lncRNAs was illustrated by the competing endogenous RNA (ceRNA) network. LncRNA ENSMUST00000128026 was predicted to bind to mmu‐miR‐6899‐3p, regulating Dnmbp expression. LncRNA NONMMUT143162.1 was predicted to competitively bind to mmu‐miR‐6899‐3p, regulating Tnip3 expression. Finally, the protein‐protein interaction (PPI) network analysis was constructed with 311 nodes and 563 edges. And the highest connectivity degrees were Mmp9, Fpr2 and Ccl3. These results provide novel insights into the functions of lncRNAs and mRNAs involved in the regulation of the intestinal epithelial barrier.

中文翻译：

---

右旋糖酐硫酸钠诱导的结肠炎肠上皮细胞相关lncRNA和mRNA表达谱


肠上皮细胞（IEC）功能障碍引起的肠上皮屏障损伤在炎症性肠病（IBD）的发病机制和发展中起着至关重要的作用。最近，一些研究表明长链非编码 RNA (lncRNA) 在 IBD 中的新兴作用。本研究的目的是揭示结肠炎小鼠模型 IEC 中的 lncRNA 和 mRNA 表达谱，并扩大我们对肠上皮屏障调节的理解。从野生型小鼠和葡聚糖硫酸钠 (DSS) 诱导小鼠的结肠中分离 IEC，用于高通量 RNA 测序。与对照组相比，DSS组共检测到254个上调和1013个下调的mRNA，以及542个上调和766个下调的lncRNA。通过实时定量 PCR 验证了四种 mRNA 和六种 lncRNA。功能分析显示失调的mRNA参与TLR7信号通路、IL-1受体活性、BMP受体结合和IL-17信号通路。此外，竞争性内源RNA（ceRNA）网络说明了差异表达的mRNA和lncRNA之间存在间接相互作用的可能性。 LncRNA ENSMUST00000128026 预计会与 mmu-miR-6899-3p 结合，调节 Dnmbp 表达。 LncRNA NONMMUT143162.1 预计会竞争性结合 mmu-miR-6899-3p，调节 Tnip3 表达。最后，构建了具有 311 个节点和 563 个边的蛋白质-蛋白质相互作用（PPI）网络分析。连接度最高的是Mmp9、Fpr2和Ccl3。这些结果为参与肠上皮屏障调节的 lncRNA 和 mRNA 的功能提供了新的见解。