The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; Puro^R) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.

中文翻译：

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使用TALENs为NKX2-5生成具有双重报告基因的人iPSC品系GIBHi002-A-2

人类转录因子NKX2-5在心脏形成和发育中起着重要作用，因此可用于分离与人类多能干细胞（hPSC）分化的心肌细胞（CMs）。在这里，我们使用TALENs将增强的GFP（eGFP）和Pac（嘌呤霉素抗性基因； Puro ^R）敲入人iPSC系iPSN0003的NKX2-5的外显子1编码区。生成的GIBHi002-A-2使我们能够通过心脏祖细胞（CPC）监视和优化心脏分化过程，并分离出iPSC衍生的CM进行药物筛选。