Human FOXH1 (Forkhead Box H1) gene encodes a human homolog of Xenopus forkhead activing signal transducer-1 and has been shown to play an important role in mesendoderm formation in X. tropicalis and mice. However, little is known about the function of FOXH1 in human development. Here we generated a FOXH1 homozygous knockout human embryonic stem cell (hESC), WAe009-A-42 by CRISPR/Cas9 mediated gene targeting. The WAe009-A-42 retained a typical undifferentiated morphology and normal karyotype, pluripotenty, and trilineage differentiation potential in vivo and vitro.

中文翻译：

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通过 CRISPR/Cas9 系统生成 FOXH1 纯合敲除人胚胎干细胞系

人类 FOXH1（叉头盒 H1）基因编码爪蟾叉头激活信号转导器 1 的人类同源物，并已被证明在热带爪蟾和小鼠的中内胚层形成中发挥重要作用。然而，人们对 FOXH1 在人类发育中的功能知之甚少。在这里，我们通过 CRISPR/Cas9 介导的基因打靶产生了 FOXH1 纯合敲除人胚胎干细胞 (hESC)，WAe009-A-42。 WAe009-A-42在体内和体外保留了典型的未分化形态和正常核型、多能性和三系分化潜力。