In cartilage tissue engineering, research on materials for three-dimensional (3D) scaffold has attracted attention. Decellularized matrix can be one of the candidates for the scaffold material. In this study, decellularization of regenerated cartilage was carried out and its effectiveness as a scaffold material was examined. Three-dimensionally-cultured cartilage constructs in the differentiation medium containing IGF-1 produced more cartilage matrix than those in the proliferation medium. Detergent-enzymatic method (DEM) could decellularize 3D-cultured cartilage constructs only by 1 cycle without breaking down the structure of the constructs. In vitro, newly-seeded chondrocytes were infiltrated and engrafted into decellularized constructs in the proliferation medium, and newly formed fibers were observed around the surface where newly-seeded cells were attached. Recellularized constructs could mature similarly as those without decellularization in vivo. The decellularized 3D-cultured matrix from regenerative cartilage is expected to be used as a scaffold material in the future.

在软骨组织工程中，对三维（3D）支架材料的研究引起了人们的关注。去细胞基质可以是支架材料的候选之一。在这项研究中，进行了再生软骨的脱细胞，并检查了其作为支架材料的有效性。在包含IGF-1的分化培养基中进行三维培养的软骨构建物比在增殖培养基中生成的软骨基质更多。洗涤剂-酶法（DEM）只能在1个周期内将3D培养的软骨构建体脱细胞，而不会破坏构建体的结构。在体外，将新播种的软骨细胞浸润并植入到增殖培养基中的脱细胞构建体中，并在附着新种子细胞的表面附近观察到新形成的纤维。重新细胞化的构建体可以与没有体内脱细胞的构建体相似地成熟。来自再生软骨的脱细胞3D培养基质有望在将来用作支架材料。