The aim of this study was to explore the involvement of long noncoding RNAs (lncRNAs) during intermittent parathyroid hormone (PTH) induced cementogenesis. Expression profiles of lncRNAs and mRNAs were obtained using high-throughput microarray. Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and coding-noncoding gene coexpression networks construction were performed. We identified 190 lncRNAs and 135 mRNAs that were differentially expressed during intermittent PTH-induced cementogenesis. In this process, the Wnt signaling pathway was negatively regulated, and eight lncRNAs were identified as possible core regulators of Wnt signaling. Based on the results of microarrray analysis, we further verified the repressed expression of Wnt signaling crucial components β-catenin, APC and Axin2. Above all, we speculated that lncRNAs may play important roles in PTH-induced cementogenesis via the negative regulation of Wnt pathway.

本研究的目的是探讨长链非编码 RNA (lncRNA) 在间歇性甲状旁腺激素 (PTH) 诱导的牙骨质形成过程中的作用。使用高通量微阵列获得 lncRNA 和 mRNA 的表达谱。进行了基因本体富集分析、京都基因百科全书和基因组通路分析、编码-非编码基因共表达网络构建。我们鉴定了 190 个 lncRNA 和 135 个 mRNA，它们在间歇性 PTH 诱导的牙骨质形成过程中差异表达。在这个过程中，Wnt 信号通路被负调控，8 个 lncRNA 被鉴定为 Wnt 信号通路的可能核心调控因子。基于微阵列分析的结果，我们进一步验证了 Wnt 信号传导关键成分 β-catenin、APC 和 Axin2 的表达受到抑制。首先，