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Improving sweet potato protein gel properties through ε-(γ-glutamy)-lysine isopeptide cross-link catalyzed by transglutaminase
Food Bioscience ( IF 4.8 ) Pub Date : 2020-12-10 , DOI: 10.1016/j.fbio.2020.100828
Lawrence A. Arogundade , Tai-Hua Mu , Miao Zhang , Jing-Wang Chen , Hong-Nan Sun , Duqin Zhang

Production of sweet potato protein (SPP) gels with enhanced viscoelasticity and mechanical properties through transglutaminase (Tgase) catalyzed SPP ε-(γ-glutamy) - lysine cross-link was investigated. Tgase induced cross-link of SPP increased the protein molecular weight, and slightly reduced its gelling point. Gel viscoelasticity was significantly enhanced with the maximum storage modulus (Gʹmax) increasing from 2,990 Pa to 30,700 Pa as Tgase concentration increased from 0% to 5.0%. The exponent (zʹ) of the power law (Gʹ = kʹω), decreased from 0.024 (control SPP gel) to in the range 0.018–0.022 for the SPP ε-(γ-glutamy) - lysine cross-link (SPP – GL) gels. SPP gel network structure showed increased covalence interaction with increase in the level of Tgase induced cross-linking. SPP gel had no fracture stress but suffer deformation under little stress, while SPP – GL cross-linked gels formed with 1.6, 3.2 and 5.0% Tgase could withstand higher stress and had fracture stress and strain in the range 97.3–235.5 kNm−2 and 0.065–0.448 respectively. Tgase induced SPP ε-(γ-glutamy) - lysine cross-link enhanced SPP gel hardness, resilience and adhesiveness.



中文翻译:

转谷氨酰胺酶催化ε-(γ-谷氨酸)-赖氨酸异肽交联改善甘薯蛋白质凝胶特性

研究了通过转谷氨酰胺酶(T gase)催化的SPPε-(γ-谷氨酰胺)-赖氨酸交联生产具有增强的粘弹性和机械性能的甘薯蛋白质(SPP)凝胶。T gase诱导的SPP交联增加了蛋白质分子量,并略微降低了其胶凝点。凝胶的粘弹性与最大储能模量(G'被显著增强最大值)从2990 Pa至30700帕增加为T GASE浓度从0%提高到5.0%。指数(Z')的功法(G'=k'ω Z'从0.024(对照SPP凝胶)下降到Z'SPPε-(γ-谷氨酰胺)-赖氨酸交联(SPP – GL)凝胶的分子量范围为0.018–0.022。SPP凝胶网络结构显示出共价相互作用随着T气体诱导的交联水平的增加而增加。SPP凝胶没有断裂应力,但在很小的应力下会发生变形,而由1.6%,3.2%和5.0%T气体形成的SPP-GL交联凝胶可以承受更高的应力,并且断裂应力和应变范围为97.3-235.5 kNm -2和0.065–0.448。Ť GASE诱导SPPε-(γ-glutamy) -赖氨酸交联增强SPP凝胶硬度,回弹性和粘合性。

更新日期:2020-12-16
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