Abstract.

Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments of human eye such as trabecular outflow pathway, retina, iris and ciliary body after pre-treatment with iodine in ethanol. We also identified the challenges of applying this contrasting technique to lens, cornea, and retina and proposed alternative staining methods for these tissues. Thereby this work provides a starting point for other studies for imaging of human eyes in normal and pathological conditions using lab-based micro-CT systems.

摘要。

Micro-CT可视化可通过光学显微镜的分辨率和组织密度的估计来重建眼睛结构。而且，该方法排除了破坏性程序，并且由于开始时的相似步骤而允许进一步的组织学染色。我们已经显示了基于实验室的微型CT机用于在乙醇中碘预处理后可视化人眼的临床重要区室（如小梁流出道，视网膜，虹膜和睫状体）的可行性。我们还确定了将这种对比技术应用于晶状体，角膜和视网膜的挑战，并提出了针对这些组织的替代染色方法。因此，这项工作为使用基于实验室的微型CT系统在正常和病理条件下对人眼成像的其他研究提供了起点。