To identify spotted fever group (SFG) rickettsiae among ticks collected by dragging at eight sites in three provinces of the midwestern region of the Republic of Korea (ROK), genus- and species-specific quantitative real-time PCR (qPCR) assays and sequencing were performed. DNA was extracted from a total of 2,312 ticks that were assayed individually (n=140) or in pools (n=444), resulting in a total of 584 individual and pooled tick samples. The 584 tick samples were screened with the genus-specific qPCR assay (Rick17b) and produced 265 (45.38%) positive reactions [individual (n=64) and pooled (n=101) samples]. Of these genus-specific positive samples, 57 (21.51%) were identified as Candidatus Rickettsia longicornii and 48 (18.11%) were identified as R. monacensis by species-specific qPCR assays. Subsequently, nested PCR (nPCR) was performed with 120 samples, which tested positive samples for genus-specific, but not species-specific, qPCR assays. The sequences of ompA and ompB genes showed how many close relatedness to Ca. R. longicornii and Ca. R. jingxinensis isolate Xian Hl-79, uncultured Rickettsia sp. Y27-1, Ca. R. tasmanensis strain T152, R. endosymbiont of H. longicornis tick 47, and R. koreansis strain CNH17-7. In conclusion, we successfully detected specific rickettsial agents using qPCR and a sequence-based analysis approach that demonstrated the prevalence of various tick-borne Rickettsia spp. in midwestern ROK.

为了鉴定拖拉大韩民国中西部三个地区（ROK）三个省的8个地点采集的tick中的斑点热病立克次体，属和种特异性定量实时PCR（qPCR）分析和测序被执行。从总共2,312个tick中提取DNA，这些individually被单独（n = 140）或合并（n = 444）进行分析，总共产生584个单独的和合并的tick样品。使用属特异性qPCR分析（Rick17b）筛选了584个滴答样本，并产生了265个（45.38％）阳性反应[单个（n = 64），并合并了（n = 101）样本]。这些属特异性阳性样品，57（21.51％）被确定为的暂定立克longicornii和48（18.11％）被确定为R. monacensis通过物种特异性qPCR分析。随后，对120个样品进行了巢式PCR（nPCR），测试了阳性样品的属特异性而非种特异性qPCR分析。ompA和ompB基因的序列显示出与Ca有多少密切的相关性。R. longicornii和Ca. R. jingxinensis分离株西安Hl-79，未培养的立克次体菌。Y27-1，Ca的 塔斯曼河杆菌菌株T152，R . H. longicornis壁虱共生共生壁虱47和韩国R.CNH17-7菌株。总之，我们使用qPCR和基于序列的分析方法成功地检测了特定的立克次体药物，该方法证明了各种tick传立克次体spp的流行。在韩国中西部。