The composition of biomass determines its suitability for different applications within a biorefinery system. The proportion of the major biomass fractions (sugar, cellulose, hemicellulose and lignin) may vary in different sugarcane genotypes and growth environments and different parts of the plant. This study investigated the composition of mature and immature internodes, roots and mature leaves of sugarcane. Internodes were found to have a significantly larger alcohol-soluble component than leaves and roots. The primary difference between the immature and mature internodes was the ratio of soluble sugars. In mature tissues, sucrose content was significantly higher, whereas in immature internodal tissues there was lower sucrose and heightened concentrations of reducing sugars. Carbon (C) partitioning in leaf tissues was characterised by low levels of soluble components and high “other” and cell wall fractions. Root tissue had low ratios of soluble fractions relative to their cell wall contents, indicating a lack of storage of soluble carbon. There was no significant difference in the ratio of the major cell wall fractions between the major organ types. Characterisation of individual non-cellulosic monomers indicated leaf and root tissues had significantly higher arabinose and galactose fractions. Significantly larger proportions of syringyl lignin compounds and the hydroxycinnamic compound, p-coumaric acid were observed in mature internodal tissues compared to the other tissue types. Tissue-specific differences in composition were shown to greatly affect the recalcitrance of the cell wall to enzymatic saccharification. Overall, this study displayed clear evidence of the differential partitioning of C throughout the sugarcane plant in specific organs. These organ-specific differences have major implications in their utility as a bioproduct feedstock. For example, the inclusion of trash (leaves) with the culms (internodes) may alter processing efficiency.

中文翻译：

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叶片，节间和根部甘蔗生物量组成的变化和酶促糖化

生物质的组成决定了其对生物精炼系统内不同应用的适用性。主要生物质部分（糖，纤维素，半纤维素和木质素）的比例可能因甘蔗基因型和生长环境以及植物的不同部位而异。本研究调查了甘蔗的成熟和未成熟节间，根和成熟叶的组成。发现节间比叶和根具有明显更大的醇溶性成分。未成熟节和成熟节之间的主要区别是可溶性糖的比例。在成熟的组织中，蔗糖含量明显更高，而在未成熟的节间组织中，蔗糖含量较低，还原糖浓度较高。叶片组织中的碳（C）分配特征是可溶性成分含量低，“其他”和细胞壁含量高。根组织相对于其细胞壁含量而言可溶部分的比率低，表明缺乏可溶碳的储存。在主要器官类型之间，主要细胞壁部分的比例没有显着差异。各个非纤维素单体的特征表明叶和根组织具有明显更高的阿拉伯糖和半乳糖部分。与其他组织类型相比，在成熟的结节间组织中观察到明显更多的丁香基木质素化合物和羟基肉桂酸化合物对香豆酸。组成的组织特异性差异显示出极大地影响了细胞壁对酶促糖化的抵抗性。总体而言，这项研究显示了在整个甘蔗植物中特定器官中碳的差异分配的明确证据。这些特定于器官的差异对它们作为生物产品原料的实用性具有重大影响。例如，在茎（节间）中包含垃圾（叶）可能会改变处理效率。