Detection of RNA viruses from influenza and HIV to Ebola and SARS-CoV-2: a review,Analytical Methods

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Detection of RNA viruses from influenza and HIV to Ebola and SARS-CoV-2: a review
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-7 , DOI: 10.1039/d0ay01886d
Rostislav Bukasov _{1,

2,

3,

4,

5} , Dina Dossym _{1,

2,

3,

4,

5} , Olena Filchakova _{2,

3,

4,

5,

6}

Affiliation

RNA-based viruses likely make up the highest pandemic threat among all known pathogens in about the last 100 years, since the Spanish Flu of 1918 with 50 M deaths up to COVID-19. Nowadays, an efficient and affordable testing strategy for such viruses have become the paramount target for the fields of virology and bioanalytical chemistry. The detection of the viruses (influenza, hepatitis, HIV, Zika, SARS, Ebola, SARS-CoV-2, etc.) and human antibodies to these viruses is described and tabulated in terms of the reported methods of detection, time to results, accuracy and specificity, if they are reported. The review is focused, but not limited to publications in the last decade. Finally, the limits of detection for each representative publication are tabulated by detection methods and discussed. These methods include PCR, lateral flow immunoassays, LAMP-based methods, ELISA, electrochemical methods (e.g., amperometry, voltammetry), fluorescence spectroscopy, AFM, SPR and SERS spectroscopy, silver staining and CRISPR-Cas based methods, bio-barcode detection, and resonance light scattering. The review is likely to be interesting for various scientists, and particularly helpful with information for establishing interdisciplinary research.

中文翻译：

从流感和HIV到埃博拉和SARS-CoV-2的RNA病毒检测：综述

自1918年西班牙流感爆发以来，基于RNA的病毒可能构成了所有已知病原体中最大的大流行威胁，自1918年西班牙流感以来，死亡人数高达5000万，直至COVID-19。如今，针对此类病毒的有效且负担得起的测试策略已成为病毒学和生物分析化学领域的首要目标。检测病毒（流感，肝炎，艾滋病毒，寨卡病毒，SARS，埃博拉病毒，SARS-CoV-2等））和针对这些病毒的人类抗体，并根据已报告的检测方法，检测时间，准确性和特异性（如果已报告）进行了制表。审查的重点是但不限于最近十年的出版物。最后，通过检测方法将每种代表性出版物的检出限制成表格并进行讨论。这些方法包括PCR，侧向免疫测定，基于LAMP的方法，ELISA，电化学方法（例如，安培法，伏安法），荧光光谱，AFM，SPR和SERS光谱，银染和CRISPR-Cas方法，生物条形码检测，和共振光散射。这篇综述对于各个科学家来说可能是有趣的，并且对于建立跨学科研究的信息特别有帮助。

更新日期：2020-12-21

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