The pyramided genetically modified (GM) soybean [Glycine max L. (Merr.)] MON87751 × MON87708 × MON87701 × MON89788, expressing Cry1A.105, Cry2Ab2, and Cry1Ac from Bacillus thuringiensis Berliner, was approved for commercial use in Brazil. We conducted laboratory, greenhouse, and field studies to assess the efficacy of this Bt soybean against key soybean lepidopteran pests. Neonates of Anticarsia gemmatalis (Hübner) (Lepidoptera: Erebidae), Chrysodeixis includens (Walker), and Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) were exposed to Bt proteins in diet-overlay bioassays. MON87751 × MON87708 × MON87701 × MON89788 soybean and individual components were evaluated in laboratory (leaf disc), greenhouse (high artificial infestations), and in field conditions (natural infestations). Neonates of A. gemmatalis, C. includens, and H. armigera were highly susceptible to Cry1A.105 (LC50 from 0.79 to 48.22 ng/cm2), Cry2Ab2 (LC50 from 1.24 to 8.36 ng/cm2), and Cry1Ac (LC50 from 0.15 to 5.07 ng/cm2) in diet-overlay bioassays. In laboratory leaf disc bioassays and greenhouse trials, MON87751 × MON87708 × MON87701 × MON89788 soybean as well as the individual components were highly effective in controlling A. gemmatalis, C. includens, and H. armigera. Similarly, under field conditions, the pyramided genotypes expressing Cry1A.105, Cry2Ab2, and Cry1Ac were highly effective at protecting soybean against C. includens. We concluded that the individual Bt proteins expressed by GM soybean MON87751 × MON87708 × MON87701 × MON89788 killed all or nearly all the susceptible A. gemmatalis, C. includens, and H. armigera, fulfilling one important criterion for successfully delaying resistance to pyramided Bt crops.

中文翻译：

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表达 Cry1A.105、Cry2Ab2 和 Cry1Ac 蛋白的转基因大豆对巴西主要鳞翅目害虫的性能

金字塔型转基因 (GM) 大豆 [Glycine max L. (Merr.)] MON87751 × MON87708 × MON87701 × MON89788，表达来自 Bacillus thuringiensis Berliner 的 Cry1A.105、Cry2Ab2 和 Cry1Ac，已获准在巴西商业使用。我们进行了实验室、温室和田间研究，以评估这种 Bt 大豆对主要大豆鳞翅目害虫的功效。Anticarsia gemmatalis (Hübner) (Lepidoptera: Erebidae)、Chrysodeixis includens (Walker) 和 Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) 在饮食覆盖生物测定中暴露于 Bt 蛋白。MON87751 × MON87708 × MON87701 × MON89788 大豆和单个成分在实验室（叶盘）、温室（高人工侵染）和田间条件（自然侵染）中进行评估。A. gemmatalis、C. includens 和 H. 棉铃虫对 Cry1A.105（LC50 从 0.79 到 48.22 ng/cm2）、Cry2Ab2（LC50 从 1.24 到 8.36 ng/cm2）和 Cry1Ac（LC50 从 0.15 到 5.07 ng/cm2）高度敏感。在实验室叶盘生物测定和温室试验中，MON87751 × MON87708 × MON87701 × MON89788 大豆以及单个成分在控制 A. gemmatalis、C. includens 和 H. armigera 方面非常有效。类似地，在田间条件下，表达 Cry1A.105、Cry2Ab2 和 Cry1Ac 的金字塔基因型在保护大豆免受 C. includens 侵害方面非常有效。我们得出结论，转基因大豆 MON87751 × MON87708 × MON87701 × MON89788 表达的单个 Bt 蛋白杀死了所有或几乎所有易感的 A. gemmatalis、C. includens 和 H. armigera，