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Improving the Phenotypic Properties of the Ceratitis capitata (Diptera: Tephritidae) Temperature-Sensitive Lethal Genetic Sexing Strain in Support of Sterile Insect Technique Applications
Journal of Economic Entomology ( IF 2.2 ) Pub Date : 2020-10-06 , DOI: 10.1093/jee/toaa220
Mitzy F Porras 1 , Jose S Meza 1 , Edwin G Rajotte 2 , Kostas Bourtzis 1 , Carlos Cáceres 1
Affiliation  

Abstract The genetic sexing strain (GSS) of the Mediterranean fruit fly (Ceratitis capitata (Wiedemann)) Vienna 8D53− is based on a male-linked translocation system and uses two selectable markers for male-only production, the white pupae (wp) and the temperature sensitivity lethal (tsl) genes. In this GSS, males emerge from brown pupae and are resistant to high temperatures while females emerge from white pupae, are sensitive to high temperatures. However, double homozygous females (wp tsl/wp tsl) exhibit a slower development rate compared to heterozygous males (wp+tsl+/wp tsl) during the larval stage, which was attributed to the pleiotropic effects of the tsl gene. We present the first evidence that this slower development is due to a different gene, here namely slow development (sd), which is closely linked to the tsl gene. Taking advantage of recombination phenomena between the two loci, we report the isolation of a novel temperature sensitivity lethal strain using the wp mutation as a morphological marker, which showed faster development (wp tsl FD) during the larval stage and increased in its temperature sensitivity compared with the normal tsl strain. Moreover, the introgression of this novel wp tsl FD combined trait into the Vienna 8D53− GSS, resulted in a novel Vienna 8D53− FD GSS, where females showed differences in the thermal sensibility, larval development speed, and productivity profiles. The modification of these traits and their impact on the mass rearing of the GSS for sterile insect technique applications are discussed.

中文翻译:

改善头螟(双翅目:实蝇科)的表型特性,支持不育昆虫技术应用的温度敏感致死遗传性别鉴定菌株

摘要 地中海果蝇 (Ceratitis capitalta (Wiedemann)) Vienna 8D53− 的遗传性别鉴定品系 (GSS) 基于雄性连锁易位系统,并使用两种选择性标记仅用于雄性生产,白蛹 (wp) 和温度敏感性致死 (tsl) 基因。在这个GSS中,雄性从棕色蛹中出现并且耐高温,而雌性从白色蛹中出现,对高温敏感。然而,在幼虫阶段,双纯合雌性 (wp tsl/wp tsl) 表现出比杂合雄性 (wp+tsl+/wp tsl) 更慢的发育速度,这归因于 tsl 基因的多效性。我们提出了第一个证据表明这种较慢的发育是由于不同的基因造成的,这里称为缓慢发育 (sd),它与 ​​tsl 基因密切相关。利用两个基因座之间的重组现象,我们报告了使用 wp 突变作为形态标记物分离出一种新的温度敏感性致死菌株,该菌株在幼虫阶段显示出更快的发育 (wp tsl FD) 并且其温度敏感性增加与正常的 tsl 应变。此外,这种新型 wp tsl FD 组合性状渗入到 Vienna 8D53− GSS 中,产生了新型 Vienna 8D53− FD GSS,其中雌性在热敏感性、幼虫发育速度和生产力方面表现出差异。讨论了这些性状的改变及其对用于昆虫不育技术应用的 GSS 大规模饲养的影响。我们报告了使用 wp 突变作为形态标记的新型温度敏感性致死菌株的分离,与正常 tsl 菌株相比,其在幼虫阶段显示出更快的发育(wp tsl FD)并且其温度敏感性增加。此外,这种新型 wp tsl FD 组合性状渗入到 Vienna 8D53− GSS 中,产生了新型 Vienna 8D53− FD GSS,其中雌性在热敏感性、幼虫发育速度和生产力方面表现出差异。讨论了这些性状的改变及其对用于昆虫不育技术应用的 GSS 大规模饲养的影响。我们报告了使用 wp 突变作为形态标记的新型温度敏感性致死菌株的分离,与正常 tsl 菌株相比,其在幼虫阶段显示出更快的发育(wp tsl FD)并且其温度敏感性增加。此外,这种新型 wp tsl FD 组合性状渗入到 Vienna 8D53− GSS 中,产生了新型 Vienna 8D53− FD GSS,其中雌性在热敏感性、幼虫发育速度和生产力方面表现出差异。讨论了这些性状的改变及其对用于昆虫不育技术应用的 GSS 大规模饲养的影响。这种新型 wp tsl FD 组合性状渗入到 Vienna 8D53− GSS 中,产生了新型 Vienna 8D53− FD GSS,其中雌性在热敏感性、幼虫发育速度和生产力方面表现出差异。讨论了这些性状的改变及其对用于昆虫不育技术应用的 GSS 大规模饲养的影响。这种新型 wp tsl FD 组合性状渗入到 Vienna 8D53− GSS 中,产生了新型 Vienna 8D53− FD GSS,其中雌性在热敏感性、幼虫发育速度和生产力方面表现出差异。讨论了这些性状的改变及其对用于昆虫不育技术应用的 GSS 大规模饲养的影响。
更新日期:2020-10-06
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