ABSTRACT

This research aimed to validate real-time polymerase chain reaction using specific primer targeting on cytochrome-b 33 (cyt-b 33). The primer cyt-b 33 could specifically amplify dog meat DNA and method could detect 0.5 pg dog meat DNA in meatball formulation. The repeatability assay was acceptable with RSD value ≤ 25%. The validated method was further used for detection of meatball products commercially sold in Yogyakarta Indonesia, and results revealed that all samples (n = 14) did not contain dog meat DNA. This research finding supports the implementation of Indonesian Law Number 33 of 2014 in developing method for Halal Product Assurance.

摘要

这项研究旨在使用针对细胞色素b 33（cyt-b 33）的特异性引物验证实时聚合酶链反应。cyt-b 33引物可特异性扩增狗肉DNA，该方法可检测肉丸配方中的0.5 pg狗肉DNA。RSD值≤25％的重复性测定是可以接受的。经验证的方法还用于检测在印度尼西亚日惹市售的肉丸产品，结果表明，所有样品（n = 14）均不含狗肉DNA。该研究结果支持在开发清真产品保证方法中实施2014年第33号印尼法律。