Abstract

Phthalates that people are exposed to every day are toxic carcinogenic chemicals with proven harmful effects on growth and reproduction. Ellagic acid (EA) is a polyphenol derivative known for its antioxidant properties. We hypothesized that the possible reproductive damage mechanism of phthalates is oxidative attack and ellagic acid could have a protective effect against radical forms in the body through its antioxidant properties. Thirty-two male rats were randomly divided into 4 groups, with 8 rats in each. Phthalate (DBP) was administered intraperitoneally and EA acid through gastric oral gavage (phthalate group 500 mg/kg/day DBP; EA group 2 mg/kg/day ellagic acid; the treatment group 500 mg/kg/day DBP and 2 mg/kg/day EA). The vehicle of DBP and EA, carboxymethyl cellulose was administered to control group. At the end of 4 weeks the testis tissue samples were taken under mild anesthesia. Tissue malondialdehyde, antioxidant parameters, sperm motility, sperm density and abnormal spermatozoon ratios were determined. Analysis was performed with One Way ANOVA test using SPSS 12.0 program. As a result; it has been shown that DBP causes oxidative damage by increasing the malondialdehyde level and decreasing antioxidant parameters, increased abnormal sperm rate and decreased sperm motility and concentration and histopathological damage so this damage is inhibited by the antioxidant activity of ellagic acid.

摘要

人们每天接触的邻苯二甲酸盐是有毒的致癌化学物质，已证明对生长和繁殖具有有害作用。鞣花酸（EA）是一种多酚衍生物，以其抗氧化性能而闻名。我们假设邻苯二甲酸盐的可能的生殖损伤机制是氧化攻击，鞣花酸可以通过其抗氧化特性对体内的自由基形式起保护作用。将32只雄性大鼠随机分为4组，每组8只。腹膜内给予邻苯二甲酸盐（DBP），通过胃管灌胃EA酸（邻苯二甲酸盐组500 mg / kg /天DBP； EA组2 mg / kg /天鞣花酸；治疗组500 mg / kg /天DBP和2 mg /千克/天EA）。对照组给予DBP和EA的媒介，羧甲基纤维素。在4周结束时，在轻度麻醉下采集睾丸组织样品。测定组织丙二醛，抗氧化剂参数，精子活力，精子密度和异常精子比率。使用SPSS 12.0程序通过单向方差分析进行分析。结果是; 已经表明，DBP通过增加丙二醛水平和降低抗氧化剂参数，提高精子异常率和降低精子活力和浓度以及组织病理学损伤而引起氧化损伤，因此鞣花酸的抗氧化活性可以抑制这种损伤。