We present a microscope on chip for automated imaging of Drosophila embryos by light sheet fluorescence microscopy. This integrated device, constituted by both optical and microfluidic components, allows the automatic acquisition of a 3D stack of images for specimens diluted in a liquid suspension. The device has been fully optimized to address the challenges related to the specimens under investigation. Indeed, the thickness and the high ellipticity of Drosophila embryos can degrade the image quality. In this regard, optical and fluidic optimization has been carried out to implement dual‐sided illumination and automatic sample orientation. In addition, we highlight the dual color investigation capabilities of this device, by processing two sample populations encoding different fluorescent proteins. This work was made possible by the versatility of the used fabrication technique, femtosecond laser micromachining, which allows straightforward fabrication of both optical and fluidic components in glass substrates.

中文翻译：

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利用芯片上的光片荧光显微镜对果蝇胚胎进行自动成像

我们提出了一种芯片上的显微镜，用于通过光片荧光显微镜对果蝇胚胎进行自动成像。这种由光学和微流体组件组成的集成设备，可以自动获取在液体悬浮液中稀释的标本的3D图像堆栈。该设备已经过全面优化，以应对与正在研究的标本有关的挑战。确实，果蝇的厚度和高椭圆率胚胎会降低图像质量。在这方面，已经进行了光学和流体优化，以实现双面照明和自动样品定向。此外，我们通过处理两个编码不同荧光蛋白的样本群体，突出了该设备的双色调查功能。飞秒激光微加工是所用制造技术的多功能性，使得这项工作成为可能。飞秒激光微加工可以在玻璃基板中直接制造光学和流体组件。