The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia‐like cell derivative approaches,STEM CELLS Translational Medicine

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The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia‐like cell derivative approaches
STEM CELLS Translational Medicine ( IF 5.4 ) Pub Date : 2020-12-09 , DOI: 10.1002/sctm.20-0127
Andreas Bruzelius _{1,

2} , Isabel Hidalgo _{1,

3} , Antonio Boza-Serrano _{4,

5} , Anna-Giorgia Hjelmér ₁ , Amelie Tison ₁ , Tomas Deierborg ₅ , Johan Bengzon ₁ , Tania Ramos-Moreno _{1,

5}

Affiliation

Microglia, the immune sentinel of the central nervous system (CNS), are generated from yolk sac erythromyeloid progenitors that populate the developing CNS. Interestingly, a specific type of bone marrow‐derived monocyte is able to express a yolk sac microglial signature and populate CNS in disease. Here we have examined human bone marrow (hBM) in an attempt to identify novel cell sources for generating microglia‐like cells to use in cell‐based therapies and in vitro modeling. We demonstrate that hBM stroma harbors a progenitor cell that we name stromal microglial progenitor (STR‐MP). STR‐MP single‐cell gene analysis revealed the expression of the consensus genetic microglial signature and microglial‐specific genes present in development and CNS pathologies. STR‐MPs can be expanded and generate microglia‐like cells in vitro, which we name stromal microglia (STR‐M). STR‐M cells show phagocytic ability, classically activate, and survive and phagocyte in human brain tissue. Thus, our results reveal that hBM harbors a source of microglia‐like precursors that can be used in patient‐centered fast derivative approaches.

中文翻译：

人骨髓含有一个 CD45- CD11B+ 细胞祖细胞，允许快速的小胶质细胞样细胞衍生方法

小胶质细胞是中枢神经系统 (CNS) 的免疫哨兵，由发育中的 CNS 中的卵黄囊红髓细胞祖细胞产生。有趣的是，一种特定类型的骨髓衍生单核细胞能够表达卵黄囊小胶质细胞特征并在疾病中填充 CNS。在这里，我们检查了人类骨髓 (hBM)，试图确定用于产生小胶质细胞样细胞的新细胞来源，以用于基于细胞的治疗和体外建模。我们证明 hBM 基质含有一个祖细胞，我们将其命名为基质小胶质细胞祖细胞 (STR-MP)。STR-MP 单细胞基因分析揭示了发育和 CNS 病理学中存在的共有遗传小胶质细胞特征和小胶质细胞特异性基因的表达。STR-MPs 可以在体外扩增并产生小胶质细胞样细胞，我们将其命名为基质小胶质细胞 (STR-M)。STR-M 细胞表现出吞噬能力，经典激活，并在人脑组织中存活和吞噬细胞。因此，我们的结果表明，hBM 含有可用于以患者为中心的快速衍生方法的小胶质细胞样前体来源。

更新日期：2020-12-09

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